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2025-04-25
This study uncovers the transcriptional regulatory network controlling SmCYP71D375, a key enzyme involved in tanshinone biosynthesis in Salvia miltiorrhiza. Using the promoter of SmCYP71D375 as bait in a yeast one-hybrid screen, researchers identified SmMYB53, an R2R3-MYB transcription factor, as an upstream regulator. Overexpression of SmMYB53 in transgenic hairy roots increased SmCYP71D375 expression and promoted tanshinone accumulation, while RNAi-mediated silencing of SmMYB53 reduced it.
2025-04-22
A Comprehensive Overview – Dual-Luciferase Reporter Gene Assay
The dual-luciferase reporter gene assay employs Firefly luciferase as the reporter gene and Renilla luciferase as the internal control gene. By simultaneously detecting the activity of both luciferases, this system enables accurate quantitative analysis of target gene expression or molecular interactions.
2025-04-18
Porcine reproductive and respiratory syndrome virus (PRRSV) is a single-stranded positive-sense RNA virus with an envelope. It-encoded non-structural protein 1α (nsp1α) plays a key role in evading host immune responses. Exploring the interaction between host factors and PRRSV nsp1α is crucial for understanding the mechanism of virus immune escape and virus control. Here, we constructed a cDNA library using porcine lung tissues and identified 33 potential host proteins interacting with viral nsp1α using yeast two-hybrid (Y2H) screening. These interactions were further analyzed using Gene Ontology and KEGG pathway analysis.
2025-04-15
A working model was proposed in which LoMYB26 interacts with LoJAZ4 to participate in JA-mediated endothecium lignification and anther dehiscence. When bioactive JA (JA-Ile, Me-JA) accumulates, LoJAZ4 is degraded, releasing the transcription factor LoMYB26. LoMYB26 directly binds to the LoCOMT promoter and activates its expression to promote lignin deposition in the anther endothecium. The secondary wall thickening of the anther endothecium results in timely anther dehiscence.
2025-04-10
A Comprehensive Guide to Transcription Factor Research Strategies (Part II)
In the first part of A Comprehensive Guide to Transcription Factor Research Strategies, Xiaoyuan focused on two key areas: the screening and identification of transcription factors, and the functional validation of transcription factors. In this follow-up, Xiaoyuan will delve deeper into the remaining two aspects: interactions between transcription factors and their target genes, and interactions between transcription factors and other proteins or transcription factors.
2025-04-08
Salt stress has a major effect on the quality and yield of crops, and many transcription factors (TFs), such as WRKY, NAC, and ERF TFs have been shown to participate in the regulation of salt stress. Squamosa promoter binding protein-like (SPL) TFs play a role in plant floral organ development, metal ion responses and disease resistance. However, the precise function of SPL TFs in regulating the salt stress in plants remains unclear. In this study, we investigated the mechanism by which SPL TFs regulate salt stress in apple (Malus domestica).
2025-04-01
A Comprehensive Guide to Transcription Factor Research Strategies (Part 1)
Proteins, as crucial products of gene expression, play a vital role in biological activities. Among the numerous proteins in living organisms, transcription factors (TFs) hold a particularly important position.
Transcription factors are a class of proteins that regulate gene transcription. They achieve this by directly or indirectly interacting with cis-regulatory elements in the promoter region of genes, thereby enhancing or reducing RNA polymerase activity, which in turn promotes or inhibits gene expression. TFs play essential roles in biological growth and development, signal transduction, and responses to biotic and abiotic stresses.
2025-03-27
In 1971, scientists proposed the famous "acid growth theory" based on the correlation between auxin, cell wall acidification (a process where the pH of the plant cell wall decreases, increasing its extensibility), and cell elongation.
The classical acid growth theory suggests that auxin promotes H+ efflux by stimulating proton pumps (PM H+-ATPase) on the plant cell membrane, leading to cell wall acidification and activation of cell wall remodeling proteins such as expansins (EXP), which in turn facilitates water uptake, cell expansion, and elongation.
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