Products

Innovative Yeast-Based And Molecular Biology Kits To Accelerate Your Life Sciences Research.

undefined
+
  • undefined

Plant Direct PCR Kit

图片名称

Price:

The Plant Direct PCR Kit by ProNet Biotech enables fast and reliable plant gene amplification with a simple protocol. Includes green dye for immediate electrophoresis, and 3' A-tail PCR products for TA cloning. Ideal for plant genomic research.

INSTRUCTION & COA:

  • Product Description
  • Product Overview

    The Plant Direct PCR Kit includes a green dye, enabling immediate electrophoresis after the reaction is completed. It is designed to amplify the target gene fragment from plants quickly, saving both time and labor. The Plant Lysis Buffer is used to break down plant cells, while the 2×Plant PCR Mix contains PCR buffer, dNTPs, MgCl₂, and heat-activated Taq enzyme, requiring only primers and template/plant lysate for amplification. This reduces pipetting steps, enhances stability, and the 2×Plant PCR Mix contains stabilizers to maintain stability after repeated freeze-thaw cycles. The PCR products have an 'A' added to the 3' end, making them suitable for TA cloning.


    Product Specifications

    Catalog No.: RY8005

    Specifications: 80T / 320T


    Storage Conditions

    Store at -20°C. Shelf life: 1 year.


    Product Components

    Component

    Unit & State

    Quantity

    2×Plant PCR Mix

    1ml/vial, liquid

    1ml

    Plant Lysis Buffer

    1ml/vial, liquid

    1ml

    Instruction Manual

    Paper version

    1


    Product Advantages

    Quick and efficient plant gene amplification

    Reduced pipetting steps, improving consistency and reliability

    Stability even after repeated freeze-thaw cycles

    Green dye for immediate electrophoresis

    3' A-tail PCR products suitable for TA cloning

    No need for complex plant tissue preparation


    Experimental Guidelines

    Plant Direct PCR Kit Experimental Guidelines

    1. Add 20 μl of Plant Lysis Buffer into a 1.5 ml EP tube, and insert a small piece of plant sample.

    2. Use a sterilized yellow pipette tip to gently grind the sample and incubate at 98°C for 5-10 minutes.

    3. Prepare a sterile PCR tube and prepare the following reaction mix:

    PCR Reaction Mix:

    Reagent

    Volume (μl)

    2×Plant PCR Mix

    25 μl

    Primer F (10 μM)

    2 μl

    Primer R (10 μM)

    2 μl

    Template*

    2 μl

    ddH₂O

    Up to 50 μl

    *After step 2, centrifuge the reaction at 12,000 rpm for 2-3 minutes, then take 2 μl as the template.

    PCR Reaction Conditions:

    Temperature

    Time

    Cycles

    98°C

    3 minutes

    1

    98°C

    15 seconds

    35-40

    Tm*

    15 seconds

    72°C

    15 seconds/kb

    72°C

    5 minutes

    1

    4°C

    1

    *Adjust annealing temperature (Tm) according to primer Tm value, typically set at Tm ± 3°C.


    Case Study

    Testing with different plant species using our PCR mix.

    Plant Direct PCR Kit Case Study

    Disclaimer: This product is intended for research purposes only. It is not intended for diagnostic or therapeutic use.

     

     

     

Any question? Get in touch with us!