The Plant Direct PCR Kit by ProNet Biotech enables fast and reliable plant gene amplification with a simple protocol. Includes green dye for immediate electrophoresis, and 3' A-tail PCR products for TA cloning. Ideal for plant genomic research.

INSTRUCTION & COA：

Consult Now

Product Description

Product Overview
The Plant Direct PCR Kit includes a green dye, enabling immediate electrophoresis after the reaction is completed. It is designed to amplify the target gene fragment from plants quickly, saving both time and labor. The Plant Lysis Buffer is used to break down plant cells, while the 2×Plant PCR Mix contains PCR buffer, dNTPs, MgCl₂, and heat-activated Taq enzyme, requiring only primers and template/plant lysate for amplification. This reduces pipetting steps, enhances stability, and the 2×Plant PCR Mix contains stabilizers to maintain stability after repeated freeze-thaw cycles. The PCR products have an 'A' added to the 3' end, making them suitable for TA cloning.
Product Specifications
Catalog No.: RY8005
Specifications: 80T / 320T
Storage Conditions
Store at -20°C. Shelf life: 1 year.
Product Components
Component
Unit & State
Quantity
2×Plant PCR Mix
1ml/vial, liquid
1ml
Plant Lysis Buffer
1ml/vial, liquid
1ml
Instruction Manual
Paper version
1
Product Advantages
Quick and efficient plant gene amplification
Reduced pipetting steps, improving consistency and reliability
Stability even after repeated freeze-thaw cycles
Green dye for immediate electrophoresis
3' A-tail PCR products suitable for TA cloning
No need for complex plant tissue preparation
Experimental Guidelines
1. Add 20 μl of Plant Lysis Buffer into a 1.5 ml EP tube, and insert a small piece of plant sample.
2. Use a sterilized yellow pipette tip to gently grind the sample and incubate at 98°C for 5-10 minutes.
3. Prepare a sterile PCR tube and prepare the following reaction mix:
PCR Reaction Mix:
Reagent
Volume (μl)
2×Plant PCR Mix
25 μl
Primer F (10 μM)
2 μl
Primer R (10 μM)
2 μl
Template*
2 μl
ddH₂O
Up to 50 μl
*After step 2, centrifuge the reaction at 12,000 rpm for 2-3 minutes, then take 2 μl as the template.
PCR Reaction Conditions:
Temperature
Time
Cycles
98°C
3 minutes
1
98°C
15 seconds
35-40
Tm*
15 seconds
72°C
15 seconds/kb
72°C
5 minutes
1
4°C
∞
1
*Adjust annealing temperature (Tm) according to primer Tm value, typically set at Tm ± 3°C.
Case Study
Testing with different plant species using our PCR mix.
Disclaimer: This product is intended for research purposes only. It is not intended for diagnostic or therapeutic use.