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Yeast One Hybrid (Gene-Centered) Vector Kit
Catalog Number
RY8009
The advanced Yeast one-hybrid assay is an experimental tool used to analyze the interaction between proteins and DNA. It can be used to study the transcriptional regulatory mechanism of genes and help people gain an in-depth understanding of the gene expression regulatory network. The tool is highly reliable and has been carefully designed and verified to be highly reliable and stable, providing accurate and reliable experimental results.
Storage precautions:
Transport at Room Temperature.
After the primers are dissolved and stored at 4°C.
Activating the strain on the YPDA medium immediately upon receipt is recommended.
For long-term storage, it is recommended to prepare a glycerol stock (in 15% glycerol) for the activated strain and store it at –80°C.
Product Description
The quality yeast one-hybrid system is a technique developed from the yeast two-hybrid to study DNA-protein interactions, and is widely used to study the regulation of gene expression in eukaryotic cells, such as identifying DNA binding sites to discover potential binding protein genes, analyzing DNA binding structural domain information, etc.
Components
Components |
Forms |
Size |
Y187 Strain |
Solid culture medium |
One plate (diameter: 15cm) |
pGADT7 Plasmid |
Liquid |
2 ug(20ul,100ng/ul) |
pHIS2 Plasmid |
Liquid |
2 ug(20ul,100ng/ul) |
pHIS2-p53 Plasmid |
Liquid |
2 ug(20ul,100ng/ul) |
pGAD53m Plasmid |
Liquid |
2 ug(20ul,100ng/ul) |
pHIS2-F |
2 ug(20ul,100ng/ul) |
2 ug(20ul,100ng/ul) |
pHIS2-R |
2 ug(20ul,100ng/ul) |
2 ug(20ul,100ng/ul) |
AD-R |
Lyophilized powder |
2 OD |
T7-F |
Lyophilized powder |
2 OD |
Experiment Process
Related product:
Yeast Colony Rapid Detection Kit Catalog No.: RY8001
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02
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