Services

Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

AI Digital Platform

Interaction Validation Platform

Poplar Genetic Transformation

Project Brief

The genetic transformation technology for poplar trees uses the stem segments and leaves of sterile poplar seedlings as the core explants. Based on the Agrobacterium-mediated method, exogenous target genes are introduced into poplar cells. Through key steps such as callus induction, adventitious bud differentiation, and rooting cultivation, stable transgenic plants are obtained. This technology enables the overexpression and knockout of target genes in poplar trees. It is a technical means for conducting gene function analysis and improving stress resistance traits in poplar trees, and provides technical support for the cultivation of high-quality poplar varieties.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	Service Period	Delivery Standard(Default delivery of resistance gene identification)
Poplar	1. Vector construction / Gene synthesis 2. Sequencing of the target gene + Vector construction 3. The target gene has been constructed into an expression vector and its sequence has been verified through sequencing.	84K	ten	1. Vector Construction: The client does not provide the vector template and does not deliver it. 2. Gene Synthesis: The client does not provide the vector template and does not deliver it.
	overexpression		4 to 6 months	The number of positive strains in the T0 generation was ≥ 7.
	Single-gene knockout		4 to 6 months	The number of positive strains in the T0 generation was ≥ 5.

Note: Other varieties can be tested*.

Case Show

1. Co-culture 2. Screening 3. Differentiation 4. Rooting

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!