PRODUCTS
Yeast Two-Hybrid (Membrane System) Vector Kit
Price:
DUAL membrane system designed to identify interactions involving integral membrane proteins or membrane-associated proteins is derived from Dualsystems Biotech AG.
INSTRUCTION & COA：
- Product Description
-
Catalog Number:
RY8010
The advantage of the Two-Hybrid (Membrane System) is that it is designed for interactions between membrane proteins, which can effectively screen and identify interactions between membrane proteins, helping to understand biological processes related to cell membranes.
Storage:
Transport at Room Temperature.
After the primers are dissolved and stored at 4°C.
Activating the strain on the YPDA medium immediately upon receipt is recommended.
For long-term storage, it is recommended to prepare a glycerol stock (in 15% glycerol) for the activated strain and store at –80°C.
Product Description:
DUAL membrane system designed to identify interactions involving integral membrane proteins or membrane-associated proteins is derived from Dualsystems Biotech AG.
Designed to detect novel interaction partners for membrane proteins, the DUAL membrane System can be used to detect pairwise protein interactions, identify novel protein interactions by cDNA library screening, investigate ternary complexes, and map interaction domains. Scientific studies involved include membrane protein-membrane protein or membrane protein-soluble protein interaction, screening of expression library, and verification of the interaction relationship between two known proteins (one of them is a membrane protein).
Components
Components
Forms
Size
NMY51 Strain
Solid culture medium
One plate (diameter: 15cm)
pPR3-N Plasmid
Liquid
2 ug（20ul,100ng/ul)
pBT3-STE Plasmid
Liquid
2 ug（20ul,100ng/ul)
pBT3-SUC Plasmid
Liquid
2 ug（20ul,100ng/ul)
pBT3-N Plasmid
Liquid
2 ug（20ul,100ng/ul)
pBT3-C Plasmid
Liquid
2 ug（20ul,100ng/ul)
pBT3-STE-OsPT2 Plasmid
Liquid
2 ug（20ul,100ng/ul)
pPR3-N-OsPP95 Plasmid
Liquid
2 ug（20ul,100ng/ul)
pPR3-N-OsPP95CT Plasmid
Liquid
2 ug（20ul,100ng/ul)
pPR3-N-F
Lyophilized powder
2 OD
pPR3-N-R
Lyophilized powder
2 OD
pBT3-SUC/STE-F
Lyophilized powder
2 OD
pBT3-SUC/STE-R
Lyophilized powder
2 OD
pBT3-N-F
Lyophilized powder
2 OD
pBT3-N-R
Lyophilized powder
2 OD
pBT3-C-F
Lyophilized powder
2 OD
pBT3-C-R
Lyophilized powder
2 OD
Primer Information:
Primer Name
Sequence
Applicable Vector
pPR3-N-F
CGGTAAAACCGGAACATTGGA
pPR3-N
pPR3-N-R
ACTTCAGGTTGTCTAACTCCT
pBT3-SUC/STE-F
TGGCATGCATGTGCTCTG
pBT3-SUC
pBT3-SUC/STE-R
GTAAGGTGGACTCCTTCT
pBT3-STE
pBT3-N-F
CAGAAGGAGTCCACCTTAC
pBT3-N
pBT3-N-R
AAGCGTGACATAACTAATTAC
pBT3-C-F
TATAAAACTCTTGTTTTCTTC
pBT3-C
pBT3-C-R
GGTGGACTCCTTCTGAATGTT
Experiment Process
Bait Vector Selection
N-terminal
C-terminal
Vector
Intracellular
Extracellular
pBT3-N
Extracellular with signal peptide
Intracellular
pBT3-SUC
Extracellular without signal peptide
Intracellular
pBT3-STE
Intracellular
Intracellular
pBT3-N or pBT3-STE
The selection of plasmids is to ensure the VP16 expression in the cell.
If both the N-terminal and C-terminal of the bait protein are located extracellularly, shorten the gene length to make C-terminus of the Bait protein appears intracellularly, and then select the corresponding vector.
If both N-terminal and C-terminal of the bait protein are located intracellularly, and there is on signal peptide, It is recommended to select pBT3-STE Vector. Because there probably is a transmembrane structure between N-terminal and C-terminal.
Related product:
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