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Pichia Pastoris Surface Display Vector Kit
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Explore the Pichia Pastoris Surface Display Kit for efficient protein expression and antibody screening. This comprehensive system includes essential components like the X33 strain, pPICZaA-Aga2p vector, and primers, enabling high-yield expression of proteins on the yeast cell surface. Ideal for protein interaction studies, antibody discovery, and research applications. Learn more and contact us for pricing.
INSTRUCTION & COA：
- Product Description
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Pichia Pastoris Surface Display Kit for Protein Expression and Antibody Screening
Product Overview
The Pichia Pastoris Surface Display Kit leverages the α-agglutinin system to efficiently display exogenous proteins on the yeast cell surface. The fusion protein is non-covalently attached to the cell wall of Pichia pastoris. After the target protein is expressed as a fusion with Aga2p, it is secreted into the extracellular space. The Aga2p subunit forms two disulfide bonds with the Aga1p subunit, and the Aga1p subunit is covalently anchored to the yeast cell wall via β-glucan, firmly displaying the protein on the surface. This robust Pichia pastoris expression system is ideal for efficient protein expression and antibody screening.
This kit includes the essential components for successful protein expression, including the X33 strain, the pPICZaA-Aga2p vector, and a positive control strain. With this complete system, researchers can easily express and display proteins on the surface of Pichia pastoris for various applications, from protein interaction studies to antibody discovery.
CAT: RY8026
Price and Instruction: Available upon inquiry
RY8026 Product Components
Component
Name
Concentration
Specification
Quantity
Plasmid
pPICZaA-Aga2p
100 ng/μL
20 μL
1 tube
Strain
X33
Inoculated Plate
1 plate
1 plate
X33-pPICZaA-Aga2p-positive
Inoculated Plate
1 plate
1 plate
Primer
5′AOX
Lyophilized
2 OD
1 tube
3′AOX
Lyophilized
2 OD
1 tube
Storage and Shipping Conditions
Plasmid: Store at -20°C; shipped at room temperature.
Strain: Store and ship at room temperature.
Primer: Store at -20°C; shipped at room temperature.
Experimental Guidelines
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
Why Choose the Pichia Pastoris Surface Display Kit?
Efficient Protein Expression: Achieve high yields of expressed proteins on the cell surface with ease.
Ideal for Antibody Screening: Perfect for screening antibodies against your target proteins.
Complete System: This kit includes all the necessary components—strain, vector, and primers—for seamless experimental setup.
Cost-Effective: Save time and resources with an all-in-one solution designed for high-quality protein expression and screening.
FAQs
Q1: What is the Pichia Pastoris surface display system used for?
A1: The Pichia Pastoris surface display system is designed for efficient protein expression and antibody screening, enabling the display of exogenous proteins on the yeast cell surface.Q2: Can this kit be used for antibody screening?
A2: Yes, this kit is perfect for antibody screening, as it allows researchers to test and identify specific antibodies against target proteins.Q3: What is the size of the target protein expressed using this system?
A3: The target protein expressed with the X33 pPICZaA-Aga2p positive control has a size of approximately 52 kDa.Q4: Why are YPG and BMMY media used in the Pichia pastoris surface display system?
A4: The reason lies in the AOX1 promoter. In the presence of glucose, the transcription of the AOX1 promoter is inhibited. However, when methanol is used as the sole carbon source, the AOX1 gene in the host strain is strongly induced, leading to a high level of expression of the target gene. Therefore, during induction, it is necessary to remove glucose from the media and supplement methanol daily to compensate for its consumption and evaporation during the induction period.Related Products
Yeast Two-Hybrid (Nuclear System) Vector Kit
Yeast Signal Peptide Detection Vector Kit
Yeast Abiotic-Stress Resistance Gene Screening Kit
Contact Us
By adding Pichia pastoris surface display to your research, you can enhance the efficiency and accuracy of your protein expression and antibody screening experiments. Whether you are studying protein-protein interactions or developing novel therapeutics, this kit provides a powerful tool to advance your scientific goals.
For further inquiries or to place an order, feel free to contact our customer service team below. We are here to assist you with your research needs.
Statement: All products are for research purposes only. Not for use in diagnostic or therapeutic applications.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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