Peptides are synthetically accessible, amenable to chemical tailoring, and have the potential to bind the typically shallow surfaces seen in therapeutically relevant—and historically intractable—protein–protein interactions (PPIs). Peptides can either act as natural ligands in the form of cofactors, coenzymes, and hormones, or directly interact with macromolecules including proteins, RNA, or DNA.
Yeast one-hybrid system is a technique developed from yeast two-hybrid to study DNA-protein interactions, and is widely used to study the regulation of gene expression in eukaryotic cells, such as identifying DNA binding sites to discover potential binding protein genes, analyzing DNA binding structural domain information, etc.
A technology based on yeast single hybridization was established to identify the recognition elements of transcription factors, named TF centered YIH. This technology can accurately, quickly, simply, and efficiently identify transcription factor recognition elements, and has broad application prospects in the study of protein DNA element interactions.
Saccharomyces cerevisiae belongs to eukaryote, which is closer to the expression system of plants and animals. This expression system includes the following processes: glycosylation, disulfide bond formation, and Post-translational modification of protein folding.
The yeast two-hybrid system is a research method for identifying and detecting protein interactions in living cells, which is performed in the eukaryotic model organism yeast, and is now used in several research fields because of its high sensitivity and wide applicability.
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