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Yeast Library Construction Services (SMART technology)

High-Quality SMART Yeast Library Construction Services

Boost your research with our advanced SMART yeast library construction technology. Experience unparalleled efficiency and precision tailored to global scientific research needs.

Technical Background

SMART (Switching Mechanism at 5' end of RNA Template) technology, developed by Clontech in 1996, revolutionized cDNA library construction. Originally designed for mammalian cDNA libraries, it now excels in yeast library construction. By leveraging reverse transcriptase’s template-switching activity, SMART technology enhances the efficiency of full-length cDNA cloning.

Technical principle Diagram

Why Choose SMART Technology?

Minimal RNA Required: Ideal for low-abundance gene expression analysis.

Effective Homogenization: Enriches low-abundance genes and reduces redundancy.

Streamlined Workflow: Simplifies processes for quick and reliable outcomes.

Sample Requirements

Sample type	Sample Request	Storage conditions (℃)	Conditions of Transport
Cell samples	The number of cells is greater than 1*10⁸	-80	dry ice
Animal samples	More than 2 g	-80	dry ice
Plant samples	More than 2 g	-80	dry ice
Total RNA	More than 200 μg	-80	dry ice

Service Content

We provide comprehensive SMART yeast library construction services across three systems:

Nuclear System Yeast Library (SMART Technology)
Service Content	Cycle (working days)	Delivery Content
1. RNA extraction 2. mRNA extraction	5 days	1. 4 ml of yeast library glycerol bacteria (divided into 4 tubes) 2. Yeast library plasmid (4 tubes greater than 400 ug) 3. Word document electronic version of the project report (including experimental data) 4. The library promises to achieve the following indicators: Library capacity> 1*10⁷ CFU Average insert fragment 0.8-1.5 kb (varies by species) Empty rate <5%
3. cDNA synthesis and adapter ligation (three-frame library) 4. cDNA homogenization 5. Recombination into yeast vector pGADT7 and electroporation 6. Electroporation of the obtained cDNA library into competent cells for cloning and amplification 7. Library detection and plasmid extraction 8. Data collation and analysis	17 days
9. Transcriptome sequencing, full library sequencing, GO+KEGG analysis	15 days

Membrane System Yeast Library (SMART Technology)
Service Content	Cycle (working days)	Delivery Content
1. RNA extraction 2. mRNA extraction	5 days	1. 4 ml of yeast library glycerol bacteria (divided into 4 tubes) 2. Yeast library plasmid (4 tubes greater than 400 ug) 3. Word document electronic version of the project report (including experimental data) 4. The library promises to achieve the following indicators: Library capacity> 1*10⁷ CFU Average insert fragment 0.8-1.5 kb (varies by species) Empty rate <5%
3. cDNA synthesis and adapter ligation (three-frame library) 4. cDNA homogenization 5. Recombination into yeast vector pPR3-N and electrotransformation 6. Electrotransform the obtained cDNA library into competent cells for cloning and amplification 7. Library detection and plasmid extraction 8. Data collation and analysis	17 days
9. Transcriptome sequencing, full library sequencing, GO+KEGG analysis	15 days

Yeast Stress Library (SMART Technology)
Service Content	Cycle (working days)	Delivery Content
1. RNA extraction 2. mRNA extraction	5 days	1. 4 ml of yeast library glycerol bacteria (divided into 4 tubes) 2. Yeast library plasmid (4 tubes greater than 400 ug) 3. Word document electronic version of the project report (including experimental data) 4. The library promises to achieve the following indicators: Library capacity> 1*10⁷ CFU Average insert fragment 0.8-1.5 kb (varies by species) Empty rate <5%
3. cDNA synthesis and adapter ligation (three-frame library) 4. cDNA homogenization 5. Recombination into yeast vector pYES2-NTB and electroporation 6. Electroporation of the obtained cDNA library into competent E. coli cells for cloning and amplification 7. Library detection and plasmid extraction 8. Data collation and analysis	17 days
9. Transcriptome sequencing, full library sequencing, GO+KEGG analysis	15 days

Applications of SMART Yeast Libraries

Yeast Two-Hybrid Systems for protein-protein interaction studies.

Stress-resistance gene research for agricultural improvement.

Transcriptome analysis and functional genomics.

Frequently Asked Questions (FAQs)

1. What is the typical timeline for SMART yeast library construction? What is delivered?

Timeline: 4-6 weeks.

Delivery Includes:

4 mL yeast library glycerol stocks (divided into 4 tubes).

Yeast library plasmids (4 tubes, >400 μg).

Quality Control Report:

Library capacity >1×10⁷ CFU.

Average insert size: 0.8-1.5 kb (species-dependent).

Empty vector rate: <5%.

2. Can bacterial samples be used for SMART library construction?

Unfortunately, bacterial samples cannot be used for SMART library construction. This technology is specifically designed for eukaryotic organisms.

3. What is a three-frame library?

A three-frame library ensures accurate protein-coding sequences in cDNA libraries. During cDNA insertion into the vector, the starting base may not align with the first codon base of a triplet, causing a frameshift. To address this:

Three different adaptors are designed for cDNA sequences.

These adaptors allow the inclusion of all three possible reading frames, ensuring at least one sequence encodes the target protein correctly.

4. What is the difference between nuclear and membrane yeast libraries?

Vectors:

Nuclear libraries use the pGADT7 vector.

Membrane libraries use the pPR3-N vector.

Applications:

Nuclear libraries: Suitable for single-hybrid screening, nuclear protein bait in two-hybrid systems, and three-hybrid screening.

Membrane libraries: Designed for membrane protein bait in two-hybrid screening. Special cases, such as receptor proteins on membranes, can also be studied.

Why Choose Us?

Cutting-edge technology tailored for yeast cDNA library construction.

Reliable and reproducible results with comprehensive project support.

Trusted by global researchers for scientific breakthroughs.

Contact Us

Ready to elevate your research? Contact us for a quote or consultation today!

Related Services: SMART yeast libraries are ideal for yeast two-hybrid system applications.

Statement: Our services are exclusively for research applications and are not intended for diagnostic or therapeutic use.

Keywords: SMART Yeast cDNA Library, Template-Switching Technology, Low-Abundance Gene Enrichment, Full-Length mRNA Cloning, Efficient RNA Utilization

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