Services
Providing Advanced Biological Technical Services for Researchers in Fundamental Science
Yeast Gene Knockout Service
Yeast Gene Knockout Service Based on Homologous Recombination
Overview
ProNet Biotech has developed an innovative homologous recombination-based gene knockout platform, optimized for Saccharomyces cerevisiae and other yeast strains. By refining vector design and transformation workflows, our method significantly improves knockout efficiency and specificity compared to traditional strategies. This service is ideal for studying gene function, regulatory networks, and metabolic engineering in yeast systems.
Technical Background
Homologous recombination is a natural DNA repair mechanism in yeast that enables precise integration of foreign DNA at a target locus. Our approach involves designing a recombination vector containing homologous arms flanking a selectable marker (e.g., antibiotic resistance gene). Upon transformation, the cassette replaces the endogenous gene via homologous recombination, achieving stable gene disruption.
Workflow
Step | Description | Working Days |
|---|---|---|
1. Phenotype Verification | Streaking yeast on selective media | 5 |
2. Gene Synthesis / Plasmid QC | Synthesis of target gene or sequencing verification | 10 |
3. Gene Knockout Procedure | Recombination cassette construction, transformation, colony PCR, sequencing confirmation, positive clone expansion, result validation | 30 |
Total Estimated Turnaround | 45 days | |
Service Deliverables
Glycerol stocks and/or agar plates of verified knockout strains
Sanger sequencing data of knockout site
One electronic experimental report
Key Advantages
High Specificity: Homologous recombination enables precise gene targeting without off-target effects.
Stable Integration: The knockout is stably inherited, suitable for long-term experiments.
Streamlined Process: Our optimized vectors and transformation strategy reduce time and complexity.
Scalable Solution: Ideal for both single-gene and high-throughput gene disruption studies.
Application Areas
Functional genomics and gene essentiality studies
Metabolic pathway engineering
Synthetic biology and strain optimization
Regulatory network dissection
Sample Requirements
To initiate the service, please provide:
Yeast strain: Clearly identified host strain for transformation
Target gene information: DNA sequence or plasmid carrying the target gene
Selectable marker: Indicate your preferred selection system (e.g., KanR, NatR)
Representative Data
Our reports include phenotypic screening plate photos (e.g., YPDA + G418), colony PCR validation (gel electrophoresis), and sequence alignment maps confirming precise gene knockout. These datasets allow clear traceability and verification.
FAQs
Q: What type of yeast strains do you support for gene knockout?
A: We primarily support Saccharomyces cerevisiae, but we can also work with other yeast species upon request. Please contact us with your strain information for feasibility evaluation.
Q: Can I provide my own yeast strain instead of BY4741?
A: Yes. Please provide strain background and relevant antibiotic sensitivities for compatibility evaluation.
Q: Do you support multiple gene knockouts or multiplex editing?
A: Yes, multi-gene knockouts can be customized upon request.
Ordering Information
For pricing, custom project assessment, or quotation, please leave a message below or contact our team at info@pronetbio.com.
Important Notes: This service is intended for research use only. Not for diagnostic or therapeutic applications.
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
Related Products
Product Inquiry
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
Need more info?
Let's connect!