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Yeast Two-Hybrid (Nuclear System) Vector Kit
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Optimize your nuclear protein interaction studies with our Yeast Two-Hybrid (Nuclear System) Vector Kit (RY8008). Complete with MATa and MATα yeast strains, pGADT7/pGBKT7 vectors, and primers, this kit supports library screening and one-on-one verification using cotransformation or mating methods. Reliable controls and detailed protocols included for accurate and efficient results.
INSTRUCTION & COA：
- Product Description
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Product Overview
The Yeast Two-Hybrid (Nuclear System) Vector Kit (RY8008) is specifically designed for performing yeast-based nuclear protein two-hybrid library screening and one-to-one interaction validation. This kit supports both cotransformation and mating methods and includes all essential components for efficient experiments.
Key features:
Compatible with nuclear protein interaction studies.
Includes three yeast strains: AH109, Y187, and Y2HGold (AH109 and Y2HGold: MATa type, Y187: MATα type).
Positive control plasmids: pGADT7-largeT and pGBKT7-p53.
Negative control plasmids: pGADT7-largeT and pGBKT7-laminC.
Primers: T7-F, BD-R, and AD-R, supporting amplification and sequencing.CAT: RY8008
Price and Instruction: Available upon inquiry
Product Components
Component
Name
Specification
Quantity
Plasmid
pGADT7
100 ng/μL
20 μL
Plasmid
pGBKT7
100 ng/μL
20 μL
Plasmid
pGADT7-largeT
100 ng/μL
20 μL
Plasmid
pGBKT7-p53
100 ng/μL
20 μL
Plasmid
pGBKT7-laminC
100 ng/μL
20 μL
Strain
AH109
Inoculated Plate
1 plate
Strain
Y187
Inoculated Plate
1 plate
Strain
Y2HGold
Inoculated Plate
1 plate
Primer
AD-R
Lyophilized
2 OD
Primer
T7-F
Lyophilized
2 OD
Primer
BD-R
Lyophilized
2 OD
Storage and Shipping Conditions
Plasmids: Store at -20°C; shipped at room temperature.
Strains: Store and ship at room temperature.
Primers: Store at -20°C; shipped at room temperature.
Experimental Guidelines
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
FAQs
What is the difference between X-α-gal and X-gal?
X-Gal: A substrate for β-galactosidase, yielding a blue product.
X-α-gal: A substrate for α-galactosidase (MEL1) used in yeast two-hybrid systems for blue-white screening. These substrates are not interchangeable.Why use 3-AT (3-Amino-1,2,4-triazole) in the experiment?
3-AT inhibits histidine leakage expression, reducing background signals caused by mild autoactivation.
Why do yeast colonies turn red?
This phenomenon occurs due to ADE2 gene disruption, leading to intermediate accumulation in the adenine biosynthesis pathway, causing colonies to appear pink or red.
Why are screened genes located in different organelles?
Yeast verifies interaction in spatially independent systems. Two proteins may interact due to their structural compatibility, regardless of their cellular localization.
Self-prepared Materials
1. Sterile PCR tubes, tips, centrifuge tubes, shakers, incubators, and reagents like 50% PEG3350, 1M LiAc, X-α-Gal, and 3-AT.
2. (Optional from Pronetbio) Yeast media (YPDA, SD-Trp, SD-Trp/-Leu/-His), yeast plasmid extraction kits, and positive clone amplification kits.
Why Choose This Kit
Comprehensive Components: Includes all essential strains, plasmids, and primers.
Flexible Applications: Supports both cotransformation and mating methods.
Reliable Controls: Includes positive and negative controls for experimental accuracy.
User-Friendly Design: Detailed experimental guidelines and troubleshooting support provided.
Ready to Advance Your Research?
Contact us today for a detailed consultation and product quotation. Let us help you unlock new insights in protein interaction research. When inquiring about this product, please reference Product Code: RY8008.
Related Service: Yeast Two-Hybrid (Nuclear Protein) Screening Service (Service Code: SRY8008)
Statement: All products are for research purposes only. Not for use in diagnostic or therapeutic applications.
Keywords: Yeast Two-Hybrid System, Nuclear Protein Interaction Kit, pGADT7 and pGBKT7 Vectors, MATa and MATα Yeast Strains, Blue-White Screening with X-α-gal.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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