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High-Fidelity DNA Polymerase for Accurate PCR Amplification | ProNet Biotech

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Discover Pronetbio's High-Fidelity DNA Polymerase designed for precise PCR amplification. Achieve high accuracy and efficiency with ultra-high fidelity and optimized buffer systems. Perfect for gene cloning and complex DNA templates.

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  • Product Description
  • Product Description

    High-Fidelity DNA Polymerase – Precision and Efficiency Perfected

    ProNet Biotech's High-Fidelity DNA Polymerase offers exceptional accuracy and performance for PCR applications. Engineered to provide superior fidelity and amplification efficiency, this enzyme is the perfect solution for researchers demanding high precision, even with complex DNA templates.


    Key Features

    Ultra-High Fidelity: Achieves more than 80 times the fidelity of Taq DNA polymerase and 10 times that of standard Pfu DNA polymerase, ensuring highly accurate amplification for critical experiments.

    Optimized for Hot Start PCR: Includes inhibitors that effectively suppress polymerase activity and 3'→5' exonuclease activity until the reaction reaches the optimal temperature, greatly improving amplification specificity and reducing non-specific binding.

    Long Fragment Amplification: Capable of amplifying DNA fragments up to 10 kb, making it ideal for large and complex DNA templates.

    Optimized Buffer System: Features a specialized buffer system that enhances performance across a wide range of experiments and complex templates, ensuring consistent and reliable results.


    Applications

    ProNet Biotech's High-Fidelity DNA Polymerase is ideal for a variety of research and laboratory applications, including:

    · Gene Cloning

    · Complex DNA Template Amplification

    · High-Throughput Library Construction

    · PCR-based Mutation Analysis

    · DNA Sequencing Preparation


    Product Specifications

    Available in various sizes to accommodate different experimental needs:

    · 100T

    · 500T

    · 1,000T


    Shipping and Storage

    Shipping: Shipped on dry ice to ensure product integrity.

    Storage: Store at -20°C for a shelf life of 12 months.

    Handling: Avoid repeated freeze-thaw cycles to preserve enzyme activity.


    Why Choose ProNet Biotech’s High-Fidelity DNA Polymerase?

    Unmatched Accuracy: With more than 80 times the fidelity of Taq polymerase, our enzyme ensures superior performance, particularly for applications requiring high precision and sensitivity.

    Reliability: Optimized buffer systems and long-fragment amplification capabilities ensure consistent, reproducible results across diverse PCR assays.

    Versatility: Whether you're working with complex DNA templates or conducting high-throughput screening, our High-Fidelity DNA Polymerase is the versatile enzyme solution for your research.


    Customer Testimonials

    “We've been using ProNet Biotech's High-Fidelity DNA Polymerase for several high-stakes cloning projects, and the results have been exceptional. The enzyme's precision has significantly improved the efficiency of our PCR reactions.” – Dr. Jack Bao


    FAQs

    Q: What is the difference between ProNet Biotech's High-Fidelity DNA Polymerase and Taq polymerase?
    A: Our High-Fidelity DNA Polymerase offers significantly higher accuracy, achieving over 80 times the fidelity of Taq polymerase, which makes it ideal for applications requiring low error rates, such as gene cloning and sequencing.

    Q: Can this polymerase amplify large DNA fragments?
    A: Yes, it supports amplification of fragments up to 10 kb, making it perfect for complex templates that require long DNA fragment amplification.

    Q: How should I store the enzyme?
    A: Store the enzyme at -20°C. Avoid repeated freeze-thaw cycles to maintain enzyme activity and longevity.


    Contact Us

    Ready to elevate your PCR experiments? Contact us today for a detailed consultation and product quotation.

     

     

     

Any question? Get in touch with us!