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Arabidopsis Thaliana Genetic Transformation

Project Brief

Arabidopsis thaliana, commonly known as mouse-ear cress, belongs to the Cruciferae family and is a dicotyledonous plant. Genetic transformation of Arabidopsis refers to the process of introducing exogenous genes into cells through artificial means, allowing them to integrate into the Arabidopsis genome and be stably expressed. This technique enables the creation of transgenic Arabidopsis plants, which is a core method in plant molecular biology research for verifying gene functions and generating mutants.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	Service Period	Delivery Standard (Default delivery includes resistance gene identification)
arabidopsis	1. Vector construction / gene synthesis 2. Target gene sequencing + vector construction 3. The target gene has been cloned into an expression vector, with the target gene sequence verified by sequencing	Columbia	ten	1. Vector construction: If the vector template is not provided by the client, it will not be delivered. 2. Gene synthesis: If the vector template is not provided by the client, it will not be delivered.
	overexpression		2 months	T0 generation seeds (0.1 - 0.15 grams)
			4 to 5 months	T1 generation positive strains (resistant strains) ≥ 10 strains
			5 to 6 months	T1 seeds (0.1 - 0.15 grams)
			8 - 9 months	More than 5 positive strains of the T2 generation
			9 to 10 months	T2 seeds (0.1 - 0.15 grams)
			12 to 13 months	More than 5 positive strains of T3 generation
			13 - 14 months	T3 seeds (0.1 - 0.15 grams)
	Single-gene knockout		2 months	T0 generation seeds (0.1 - 0.15 grams)
			4 to 5 months	There are ≥ 3 positive strains (resistant strains) in the T1 generation.
			5 to 6 months	T1 seeds (0.1 - 0.15 grams)
			8 - 9 months	More than 3 positive strains of the T2 generation
			9 to 10 months	T2 seeds (0.1 - 0.15 grams)
			12 to 13 months	More than 3 positive strains of T3 generation
			13 - 14 months	T3 seeds (0.1 - 0.15 grams)

Note: Other varieties can be tested*.

Case Show

Explant preparation, Agrobacterium transformation, screening

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