Services
Providing Advanced Biological Technical Services for Researchers in Fundamental Science
SNP Genotyping Service
SNP Genotyping Service | High-Precision KASP Assays
Reliable and High-Throughput SNP Genotyping Solutions
At ProNet Biotech, we provide SNP genotyping services based on optimized primer design algorithms and standardized workflows. Using the KASP (Kompetitive Allele Specific PCR) platform, combined with automated experimental pipelines and a professional bioinformatics team, we deliver high-accuracy, high-throughput, and cost-effective SNP detection.
Our services cover diverse research areas such as plant breeding, disease association studies, functional gene validation, and molecular-assisted selection, supporting projects from small-scale samples (≥22) to large cohorts of thousands.
Principle of SNP Genotyping
Our platform applies competitive allele-specific PCR, a fluorescence-based technology for high-throughput genotyping.
Two allele-specific forward primers (tagged with different fluorophores) and one common reverse primer are used in PCR amplification.
Fluorescence detection (via real-time qPCR instruments) identifies genotype clusters: AA, AB, or BB.
This probe-free method is highly scalable, cost-effective, and suitable for medium-to-large genotyping projects.
Application Fields
Our SNP marker analysis is widely applied across species and research areas, including:
Plant & Crop Breeding (marker-assisted selection, variety identification, seed purity testing)
Livestock & Aquaculture (genetic diversity, trait selection, transgenic detection)
Medicinal Plants & Microorganisms (functional validation, gene mapping, molecular breeding)
Human & Animal Research (disease association studies, mutation detection)
Workflow
1. Sample Reception
2. Primer Design
3. Genomic DNA Extraction & Quantification
4. Allele-Specific PCR (KASP Assay)
5. Genotyping Detection via Fluorescence System
6. Data Analysis & Delivery
Technical Advantages
High Accuracy: ≥99.5% accuracy; reproducibility >98%
High Throughput: 384-well plate format, up to 20,000 samples per day
Cost-Effective: 40–60% lower cost compared to traditional TaqMan probe assays
Sample Requirements
DNA Samples | Tissue Samples |
Concentration: 20–50 ng/μL Volume: ≥20 μL Purity: OD260/280 = 1.8–2.0 Shipping: Ice packs | Plant leaves: 50–100 mg Animal tissues: 25–50 mg Animal cells: ≥1×10⁶ cells Whole blood (EDTA/anticoagulant): 1–2 mL Shipping: Dry ice |
Deliverables
SNP genotyping results
Excel data tables
Comprehensive experimental report
Case Results
1. Thermo 7500 platform
FAM and HEX signals analyzed by endpoint fluorescence clustering.
Genotype assignment: circles (AA), squares (BB), triangles (AB).
2. LGC SNPline – Pherastar/Omega F platform
Cluster patterns:
AA: strong FAM, weak HEX (red cluster)
GG: strong HEX, weak FAM (blue cluster)
AG: both strong FAM and HEX (green cluster)
Why Choose ProNet Biotech SNP Genotyping?
Tailored solutions for plant, animal, and microbial research
Seamless integration of wet-lab assays and bioinformatics analysis
Flexible project scale: from pilot studies to large breeding programs
Cost-efficient and publication-ready data outputs
Ready to Start Your SNP Genotyping Project?
At ProNet Biotech, we deliver accurate, high-throughput, and cost-effective SNP genotyping services to accelerate your research. Whether you are working on plant breeding, animal genetics, or disease association studies, our expert team is ready to support you from start to finish.
Contact us today to discuss your project requirements and receive a customized quote.
Statement: Our services are exclusively for research applications and are not intended for diagnostic or therapeutic use.
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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