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To provide biological high-end technical services for scientific workers in the field of basic research

Conventional Yeast Platform

Nanobody Platform

AI Screening Protein-Interactins

Conventional Molecular Biology Platform

TF-Centered Yeast One-Hybrid (Y1H-TF) Screening Service

Service Overview

A technology named TF-centered Y1H has been developed based on the yeast one-hybrid (Y1H) technique to identify the recognition elements of transcription factors. This technique allows for the accurate, rapid, simple, and efficient identification of transcription factor recognition elements and has broad application prospects in the study of protein-DNA element interactions.

Technical advantages

1. Provides comprehensive insight into transcription factor recognition elements.

2. Quickly identifies various cis-acting elements bound by a specific transcription factor.

3. Expands the application range of yeast one-hybrid (Y1H) technology, allowing for both gene-centered and TF-centered research.

4. TF-Centered Y1H can identify various elements bound by transcription factors, with a much lower false-positive rate than gene-centered Y1H.

5. The method can discover a large number of new cis-acting elements; it is simple, fast, and effective.

Service content

	Material name		Deliverables
We receive	Gene sequence		1. Sequencing results of positive clones (depending on sequencing results) 2. Project report in Word document format (including experimental data) 3. Selection of 5 motifs for genome scanning analysis 4. Bait plasmid (optional)
We provide	cDNA library
Service contents		Working days
Yeast One-Hybrid Screening (TF-Centered Y1H)	1. Gene synthesis (codon optimization) / vector construction	15
	2. Self-activation detection of bait plasmid	10
	3. Co-transformation of bait plasmid and library plasmid 4. Optimization of yeast screening conditions 5. Sequencing of screening results	20
	6. Reverse validation of screening results 7. Data compilation and analysis	5
	8. Genome scanning analysis 9. Data compilation and analysis	5

Frequently Asked Questions (FAQ)

1. Why construct a library with a random sequence of only 7bp?
For new elements, the boundaries need to be determined. The longer the insert sequence, the greater the workload in defining boundaries. This approach reduces the workload in determining the boundaries of new elements.

2. Why insert the random sequence into the SmaI restriction site?
Because its restriction sequence is CCCGGG, this sequence can serve as a marker site to recognize the insert sequence, forming CCCHNNNNNNCGGG, with CCC and GGG as marker sites.

Statement: Services sold by our company are intended for research purposes only and must not be used for diagnostic or therapeutic purposes!

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