全部

全部
产品管理
新闻资讯
介绍内容
企业网点
常见问题
企业视频
企业图册

Everyone is searching:
Nanoantibody screening

Yeast Two-Hybrid Y2H
Mb Y2H for Membrane Proteins

Yeast one-Hybrid Y1H
TF-centered YlH
Yeast Three-Hybrid Y3H

Abiotic-Stress Resistance Gene Screening

Digital Analog Library Screening

Services

Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

AI Digital Platform

Interaction Validation Platform

CRISPR-Cas12a-Based Yeast Genome Editing Service

Yeast Genome Editing with CRISPR-Cas12a – Knockout, Knock-in, and Multiplex Editing

Overview

ProNet Biotech introduces a highly flexible and efficient CRISPR-Cas12a-based yeast genome editing platform, enabling single-gene knockout, multi-gene editing, and targeted gene insertion in Saccharomyces cerevisiae and other yeast strains. Compared to traditional TALEN and ZNF technologies, the CRISPR-Cas12a system offers faster workflows, higher precision, and lower off-target effects, making it ideal for advanced functional genomics, synthetic biology, and strain engineering.

Technical Background

CRISPR/Cas12a enables genome editing through a three-step process: target recognition, cleavage, and repair. The system transcribes a pre-crRNA that is processed by Cas12a into a mature crRNA, forming a nuclease complex. This complex locates a 5'-TTN-3' PAM site, binds the target via base pairing, and performs a staggered double-strand break using its RuvC domain, generating sticky ends (4–5 nt).

Repair is triggered via two major mechanisms:

NHEJ (Non-Homologous End Joining), leading to frameshift mutations

HDR (Homology-Directed Repair), used for precise gene insertions when donor DNA with ~500–1000 bp homology arms is provided

Fig1. CRISPR/Cas12a Gene Editing Principles (Swarts DC al et, 2017)

Application Areas

Functional gene disruption and loss-of-function studies

Rapid generation of genome-edited strains for phenotype analysis or drug screening

Multi-gene pathway analysis and regulatory mechanism exploration

Metabolic engineering and industrial yeast optimization

Yeast Genome Editing Workflow

Project Type	Working Days	Key Steps
Knockout / Knock-in Editing	45 days	1. Target site design 2. Editing cassette assembly 3. Vector construction 4. Yeast transformation 5. PCR verification 6. Sequencing 7. Clone expansion 8. Final validation

Deliverables

Glycerol stocks and/or plates of edited strains

Sequencing files confirming edit accuracy

One electronic experiment report including raw data

Key Advantages

Precision Editing: High fidelity with Cas12a system, reduced off-target risks

Edit Validation: Includes colony PCR and sequencing for confirmation

Flexible Design: Accepts user-defined target sequences or provides sgRNA design

Fast Turnaround: 30–50% faster than conventional gene editing workflows

Cas12 Vector Library: Pre-validated Cas12a vectors for common yeast strains

Multi-Application Ready: From academic research to industrial strain improvement

Sample Requirements

Digital Data (Required for all projects):

· Target gene name and ID

· Full genome sequence of the yeast strain to be edited

Physical Materials (if applicable):

Sample Type	Submission Standard	Shipping Condition
Yeast strain	≥1×10⁸ CFU/mL, contamination-free	Room temp, deliver within 48 hrs
Plasmid (sgRNA vector)	≥100 ng/μL, ≥20 μL	Ice pack, within 72 hrs
Glycerol stock	≥500 μL, labeled with antibiotic resistance info	Dry ice, 1–3 days
Plate colonies	Contamination-free	Room temperature

Validation Criteria

1. PCR confirmation of edit at target site (gel image included)

2. Sanger sequencing alignment confirming edit accuracy

3. Delivery of edited strain and experimental report with original data

Representative Data

Phenotype screening images of edited strains

Electroporation plate growth results

PCR validation gel images

Sanger sequencing trace alignment files

Ordering Information

For pricing, quote requests, or custom project consultation, please email us at info@pronetbio.com or leave a message below.

References

Li ZH, Liu M, Lyu XM, Wang FQ, Wei DZ. CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae. J Basic Microbiol. 2018;58(12):1100-1104.
Li, Z. H., Liu, M., Wang, F. Q., & Wei, D. Z. (2018). Cpf1-assisted efficient genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae. Biotechnology letters, 40(8), 1253–1261.
Verwaal, R., Buiting-Wiessenhaan, N., Dalhuijsen, S., & Roubos, J. A. (2018). CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae. Yeast (Chichester, England), 35(2), 201–211.
Swiat, M. A., Dashko, S., den Ridder, M., Wijsman, M., van der Oost, J., Daran, J. M., & Daran-Lapujade, P. (2017). FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Nucleic acids research, 45(21), 12585–12598.
Chen, B. C., Chen, Y. Z., & Lin, H. Y. (2023). An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in Saccharomyces cerevisiae. Biomolecules, 13(10), 1561.

Important Notes: This service is intended for research use only. It is not for diagnostic or therapeutic purposes.

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!