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Providing Advanced Biological Technical Services for Researchers in Fundamental Science
Yeast Library Construction (In-Gate Technology)
Service Overview
At ProNet Biotech, we offer cutting-edge yeast library construction services powered by our proprietary In-Gate technology. This innovative approach builds upon SMART and Gateway methodologies, utilizing a custom-designed vector and an optimized experimental workflow to eliminate the need for BP reactions. With In-Gate, we achieve significantly higher gene integrity and fidelity compared to traditional Gateway methods.
Why Choose In-Gate Technology?
Innovative Features
1. Eliminates BP Reaction: Reduces intermediate steps, minimizing gene loss.
2. Streamlined Workflow: Optimized steps for faster processing times.
3. Superior Results: Over 40% rate of full-length gene capture, exceeding standard methods.
Key Advantages
1. High Accuracy: Ensures superior gene fidelity and integrity.
2. Efficiency: Accelerates yeast library construction by approximately 50% compared to traditional methods.
3. Versatility: Supports advanced applications such as transcription factor interaction studies.
Technical Principle
In-Gate Technology relies on homologous recombination, a proven gene-editing method widely used in:
Genetic engineering.
Bacterial artificial chromosome research.
Chromosomal studies in E. coli.
Gene therapy research and development.
This precise DNA modification method enables gene knockouts, insertions, and point mutations with unparalleled accuracy.
Sample Requirements
|
Sample Type |
Sample Requirement |
Storage Condition |
Shipment Condition |
|
Cell sample |
Cell count>1*108 |
-80℃ |
Dry ice |
|
Animal sample |
>5g |
-80℃ |
Dry ice |
|
Plant sample |
>5g |
-80℃ |
Dry ice |
|
Total RNA |
>400ug |
-80℃ |
Dry ice |
Service Offerings
We provide two types of yeast libraries with In-Gate Technology:
|
(in-Gate) Yeast membrane system library construction |
||
|
Service Content |
Working days |
Delivery material |
|
1. RNA extraction 2. mRNA extraction |
5 |
1. Yeast library glycerol stock 4 ml (divided into 4 tubes) 2. Yeast library plasmid (4 tubes > 400 μg) 3. Project report in Word document format (including experimental data) 4. The library is guaranteed to meet the following specifications:
|
|
3. cDNA synthesis 4. Ligation of cDNA with adapters 5. cDNA fractionation |
5 |
|
|
6. Recombination into yeast vector pPR3-N and electroporation 7. Electroporation of the obtained cDNA library into competent E. coli cells for clone amplification |
5 |
|
|
8. Library screening and plasmid extraction |
5 |
|
|
9. Data organization and analysis |
2 |
|
|
10. Transcriptome sequencing, full library sequencing, GO + KEGG analysis |
15 |
|
|
(in-Gate) Yeast nuclear system library construction |
||
|
Service Content |
Working days |
Delivery material |
|
1.RNA extraction 2.mRNA extraction |
5 |
1. Yeast library glycerol stock 4 ml (divided into 4 tubes) 2. Yeast library plasmid (4 tubes > 400 μg) 3. Project report in Word document format (including experimental data) 4. The library is guaranteed to meet the following specifications:
|
|
3.cDNA synthesis 4.Ligation of cDNA with adapters 5.cDNA fractionation |
5 |
|
|
6.Recombination into yeast vector pGADT7 and electroporation 7.Electroporation of the obtained cDNA library into competent E. coli cells for clone amplification |
5 |
|
|
8.Library screening and plasmid extraction |
5 |
|
|
9.Data organization and analysis |
2 |
|
|
10.Transcriptome sequencing, full library sequencing, GO + KEGG analysis |
15 |
|
Frequently Asked Questions (FAQs)
1. How does In-Gate improve over Gateway technology?
In-Gate eliminates the BP reaction step, reducing intermediate processes and gene loss. This results in higher fidelity, faster workflows, and a superior full-length gene recovery rate compared to Gateway.
2. What is the timeline for yeast library construction using In-Gate?
The process typically takes 25 working days, including RNA extraction, library construction, and data analysis.
3. What types of samples can be used?
In-Gate supports:
Cell, animal, and plant samples.
Total RNA with specific quality and storage requirements.
4. What are the primary applications of In-Gate libraries?
Transcription factor interaction studies.
Gene function analysis.
Stress-resistance gene research.
Reference Case Study
Title: An experimentally-based transcription factor interaction network in seeds of Brassica napus.
Research Subject: Brassica napus
Journal: Plant Biotechnology Journal
Publication Date: 2024
Impact Factor: 13.8
Research Overview: This study utilized In-Gate Technology to construct a transcription factor interaction library, significantly reducing library construction time by approximately 50%. Using CrY2H-seq, the study identified 805 transcription factor interaction pairs, establishing the Brassica napus interaction network BnSTINet.
Why Choose ProNet Biotech?
Proprietary Technology: In-Gate offers unmatched accuracy and efficiency.
Comprehensive Solutions: End-to-end yeast library construction services.
Global Expertise: Trusted by researchers worldwide for high-quality results.
Contact Us
Ready to elevate your research? Contact us today to learn more about our In-Gate yeast library construction services.
Statement: Services sold by our company are intended for research purposes only and must not be used for diagnostic or therapeutic purposes.
Keywords: High-Fidelity Yeast Library, Innovative In-Gate Technology, Homologous Recombination Workflow, Streamlined Gene Construction, Full-Length Gene Recovery
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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