Our service
To provide biological high-end technical services for scientific workers in the field of basic research
Two-Hybrid (Nuclear System)
Technology
The yeast two-hybrid system is a research method for identifying and detecting protein interactions in living cells, which is performed in the eukaryotic model organism yeast, and is now used in several research fields because of its high sensitivity and wide applicability.
Service process
Service content |
Working days |
1. Gene synthesis(Codon Optimization) /Vector Construction |
15d |
2. Self-activation of bait plasmid |
10 d |
3. Co-transfer Bait plasmid and library plasmid 4. Selective medium for yeast screening 5. Sanger sequence of screening results |
15 d
|
6. Verify by Rotation 7. Temporary Data Report |
5 d |
8. Next-generation sequencing 9. Complete Project Data Report |
10 d |
Total |
55 d |
Features
1. Based on NGS, delivery more positive clone sequences
2. Ensure at least 8 positive clone sequences (No Frameshift Mutation);
3. ProS-3D Structural Analysis
4. Recommended Whole Sequencing Library
Services Workflow

Online Consultation
01

Solution Matching
02

Service Contract
03

One-Stop-Services
04

Project Report
05
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Tel: +86 025-85205672

Email: info@pronetbio.com

Room 1001, Floor 10, Building 3C, Nanjing Xianlin Zhigu, No. 6 Qimin West Road, Xianlin Street, Qixia District, NanJing, Jiangsu, China, 210033

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