全部

全部
产品管理
新闻资讯
介绍内容
企业网点
常见问题
企业视频
企业图册

Everyone is searching:
Nanoantibody screening

Yeast Two-Hybrid Y2H
Mb Y2H for Membrane Proteins

Yeast one-Hybrid Y1H
TF-centered YlH
Yeast Three-Hybrid Y3H

Abiotic-Stress Resistance Gene Screening

Digital Analog Library Screening

Services

Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

AI Digital Platform

Interaction Validation Platform

Yeast Two-Hybrid Screening Service (Nuclear Protein)

Yeast Two-Hybrid (Y2H) Screening Service for Nuclear Protein Interactions

Professional Protein-Protein Interaction Analysis Using Nuclear Protein cDNA Libraries

Are you searching for reliable protein interaction analysis? Our advanced Yeast Two-Hybrid (Y2H) Screening Service (Service Code: SRY8008) delivers comprehensive protein-protein interaction analysis using our extensively validated nuclear protein cDNA libraries. With coverage of over 100 species and expert bioinformatics support, we ensure high-quality results for your research needs.

Expert Y2H Analysis: The Gold Standard in Protein Interaction Studies

The Yeast Two-Hybrid system stands as the leading method for studying protein interactions in living cells. Our service utilizes the classic Gal4 transcription factor system, featuring:

· DNA binding domain (BD)
· Transcription activation domain (AD)

When your target proteins fuse with these domains, their interaction restores transcriptional activity, enabling precise detection of protein-protein interactions.

Yeast Two-Hybrid Screening Technical Diagram

Comprehensive Nuclear Protein Library Collection

Access our extensive collection of nuclear protein cDNA libraries, featuring:

· Coverage of 100+ species

· Diverse biological processes

· Extensive functional annotations

· Regular updates with new entries

Key Service Advantages

1. Enhanced Detection Accuracy

· Dual-tier screening methodology

· Three-deficiency media (SD-Trp/-Leu/-His)

· Four-deficiency media (SD-Trp/-Leu/-His/-Ade)

· Advanced X-α-gal assays

2. Complete Documentation Package

· Detailed experimental documentation

· High-resolution result images

· Comprehensive sequencing data

· Sanger sequencing

· Next-generation sequencing (NGS)

· Professional functional annotations

3. Quality Assurance

· Frame-shift analysis for positive clone verification

· NGS-based high-throughput validation

· Multiple quality control checkpoints

4. Advanced Bioinformatics Analysis

· Professional data interpretation

· Functional annotation

· Transcription factor identification

· Kinase analysis

· Interaction strength quantification

5. Dedicated Support Services

· Expert consultation

· Project-specific guidance

· Ongoing technical support

· Result interpretation assistance

Detailed Experimental Process

Our service follows a six-phase workflow for optimal results, ensuring transparency and precise tracking of your project.

Phase	Description	Working Days
Phase 1: Construction Phase	Gene synthesis and vector construction, codon optimization	15 days
Phase 2: Bait Plasmid Transformation & Self-activation Testing	Transformation of bait plasmids into AH109 yeast strain, self-activation detection using selective media.	10 days
Phase 3: Library Screening	Transformation of nuclear protein cDNA library plasmids into AH109 yeast strain, screening on SD-TLH plates.	15 days
Phase 4: Validation of Screening Results	PCR amplification, Sanger sequencing, BLAST analysis, and verification of interactions through re-screening.	5 days
Phase 5: High-Throughput Sequencing (NGS)	Collection of all clones for NGS, bioinformatics analysis, interaction network visualization, and strength analysis.	15 days
Phase 6: Reporting and Data Delivery	Comprehensive project report including sequencing data, interaction analysis, bioinformatics results, and recommendations for downstream studies.	15 days

Total Duration: ≤ 60 Working Days

Core Deliverables

· Validated positive clone sequences (≥15 sequences based on Sanger sequencing)

· Bait plasmid (optional)

· NGS sequencing results and bioinformatics analyses

· Functional annotation (GO, KEGG) and interaction strength analysis

· Detailed experimental report in Word format, including methodologies, step-by-step experimental details, high-resolution result images, and comprehensive data interpretation.

Why Partner With Us?

1. Technical Excellence

· Experienced research team
· Advanced laboratory facilities
· Proven methodology
· Quality assurance systems

2. Customization Options

· Flexible experimental design
· Protocol adaptations
· Species-specific solutions
· Timeline adjustments

3. Comprehensive Support

· Project consultation
· Technical guidance
· Result interpretation
· Follow-up assistance

Frequently Asked Questions (FAQs) of Y2H Screening

1. What type of sequence should I provide for the bait construct in a Yeast Two-Hybrid screening project? Is there any length requirement?

We recommend providing the CDS (coding sequence) for the bait gene when performing Yeast Two-Hybrid (Y2H) library screening. There is no specific length requirement, but longer sequences may increase the chance of structural complexity or self-activation, so codon optimization is often performed before vector construction.

2. How should I submit my gene sequences to you?

Please provide your gene information in the following format:

CDS sequence in a Word document (.doc/.docx).

3. What is the purpose of the re-screening (re-testing/rotation/retransformation) validation step in Y2H? How is it performed?

Re-validation is a critical step to eliminate false positives and weak interactions. In this process, we:

· Pick positive clones from the primary screen.

· Culture and spot them on selective media: double-deficiency (SD/-Leu/-Trp), triple-deficiency (SD/-Leu/-Trp/-His), quadruple-deficiency (SD/-Leu/-Trp/-His/-Ade), and SD/-Leu/-Trp/-His/-Ade + X-α-gal. This step confirms the strength and reliability of protein-protein interactions.

4. Why do yeast colonies turn red on SD/-Leu/-Trp/-His/-Ade (quadruple dropout) plates?

Red coloration in yeast colonies on quadruple dropout media can occur due to:

· Nutrient depletion: Yeast consumes the available adenine, leading to the accumulation of metabolic intermediates like AIR (5’-phosphoribosylaminoimidazole).

· ADE2 gene mutation: Some yeast strains have defective ADE2, which results in red colony color during adenine depletion. These colonies are usually viable but grow slower, and may need to be re-cultured in fresh medium.

5. What is the difference between “small transformation” and “large transformation” in a Y2H experiment?

Small transformation refers to the introduction of the bait plasmid only into the yeast strain. This step is mainly used for:

· Evaluating transformation efficiency.

· Testing for self-activation potential.

Large transformation involves the co-transformation of the bait plasmid and the cDNA library plasmid into the yeast strain. This is the actual screening step for detecting interaction partners.

Contact Information

Ready to explore protein interactions with precision and confidence? Contact us today to discuss your project requirements and receive a customized solution. When inquiring about this Yeast Two-Hybrid (Y2H) screening service, please reference Service Code: SRY8008.

☆ Related Optional Service: Yeast Two-Hybrid One-to-One Interaction Validation

Y2H Validation Service Overview

In addition to our comprehensive Y2H Screening service, we provide a specialized Yeast Two-Hybrid One-to-One Interaction Validation service. This service is tailored for researchers seeking to confirm specific protein-protein interactions, either to validate screening results or to test predefined interaction pairs.

Y2H Validation Experimental Workflow

Our verification service includes the following stages to ensure precise and reliable results:

1. Gene Synthesis and Vector Construction (10 Working Days)

· Codon optimization and synthesis of target protein genes.

· Construction of Protein A into the pGADT7 vector and Protein B into the pGBKT7 vector.

2. Yeast Transformation and Self-activation Testing (7 Working Days)

· Transformation of recombinant plasmids into yeast cells.

· Self-activation testing using selective media to ensure experimental reliability.

3. Interaction Validation (10 Working Days)

· Setting up positive and negative controls for interaction verification.

· Confirming protein-protein interactions using selective media and X-α-gal assays.

Y2H Validation Deliverables

· Recombinant plasmids (Optional);
· A digital experiment report, including methodologies, step-by-step experimental details, high-resolution result images, and comprehensive data interpretation.

Statement: Our services are exclusively intended for research applications and must not be used for diagnostic or therapeutic purposes.

Related Product: Yeast Two-Hybrid (Nuclear System) Vector Kit [CAT：RY8008]

Related Services: Explore our Co-IP and Pull-Down Assays for advanced protein-protein interaction validation. For membrane protein-protein interaction analysis, please check Yeast Two-Hybrid Screening (Membrane Protein).

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!