Our service
To provide biological high-end technical services for scientific workers in the field of basic research
Two-Hybrid (Nuclear System)
Technology
The newest yeast two-hybrid system is a research method for identifying and detecting protein interactions in living cells, which is performed in the eukaryotic model organism yeast and is now used in several research fields because of its high sensitivity and wide applicability.
Service process
Service content |
Working days |
1. Gene synthesis(Codon Optimization) /Vector Construction |
15d |
2. Self-activation of bait plasmid |
10 d |
3. Co-transfer Bait plasmid and library plasmid 4. Selective medium for yeast screening 5. Sanger sequence of screening results |
15 d
|
6. Verify by Rotation 7. Temporary Data Report |
5 d |
8. Next-generation sequencing 9. Complete Project Data Report |
10 d |
Total |
55 d |
Features
1. Based on NGS, delivery of more positive clone sequences
2. Ensure at least 8 positive clone sequences (No Frameshift Mutation);
3. ProS-3D Structural Analysis
4. Recommended Whole Sequencing Library
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Room 1001, Floor 10, Building 3C, Nanjing Xianlin Zhigu, No. 6 Qimin West Road, Xianlin Street, Qixia District, NanJing, Jiangsu, China, 210033
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