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Plant Genetic Transformation Services

High-Efficiency Genetic Engineering Solutions for Multiple Plant Species

Service Overview

Plant genetic transformation refers to the process of introducing foreign genes or plasmids into plant cells and integrating them into the plant genome. This technique is not only fundamental for studying plant development and gene function, but also essential in modern agriculture for breeding crops with improved traits.

We offer comprehensive genetic modification services—such as gene knockout, overexpression, and precise editing (PE)—across a wide range of species, including:

Model plants: Arabidopsis thaliana, tobacco, rice

Major crops: maize, potato, soybean

Economic plants: tomato, lettuce

Forage grasses: Cynodon dactylon, Poa pratensis, Agrostis stolonifera, Zoysia japonica

Our advanced and stable transformation systems help introduce desirable traits like yield improvement, enhanced nutrition, and stress resistance. Services are fully customizable, covering project design, feasibility evaluation, execution, and training support.

Workflow

Species and vector selection

Gene synthesis and vector construction

Tissue preparation and transformation

Resistant callus screening and regeneration

PCR/Sanger sequencing for positive T0 lines

(Optional) Gene expression verification via qPCR

Plant Genetic Transformation Services Workflow

Technical Advantages

Tailored Solutions: Multidimensional services—overexpression, RNAi, CRISPR editing—with customizable strategies in vector construction, transformation, detection, and analysis.

Wide Species Coverage: Applicable to rice, soybean, maize, tomato, lettuce, tobacco, Arabidopsis, potato, and more.

Efficient Screening: Supports various eukaryotic resistance markers (Hygromycin, G418, Basta, Glyphosate, Mannose); offers PCR and Sanger sequencing reports for resistant lines.

Full Project Support: Expert pre-sale consultation, detailed project design, transparent experiment tracking, and comprehensive final reports.

Experimental Workflow & Deliverables

Service

Delivery materials

A. Selection of target genes

B. Gene synthesis / Vector construction

The transformed plasmid vector

A. Preparation of explants

B. Infection of explants

C. Callus selection

D. Callus differentiation into shoots

T0 positive seedlings

A. DNA extraction
B. Primer design for amplification

C.PCR amplification and gel electrophoresis

D. Sanger sequencing

1. PCR detection report

2. Sanger sequencing chromatogram

A. RNA extraction (optional)

B. qPCR primer design

C. Real-time PCR

D. Quantitative data analysis

1. Raw data and result images

2.Standard experimental report (including experimental steps and workflow)

Supported Species, Services, and Timeline

Species	Service type	Variety	Service period	Delivery standards
Rice	Genetic transformation	Japonica rice varieties such as Nipponbare and Zhonghua 11	2.5 to 3 months	At least 20 PCR-positive T0 plants
	Single-gene knockout			At least 10 T0 edited lines
	Multi-gene knockout			At least 10 T0 edited lines
	Genetic transformation	Indica rice varieties such as Huanghuazhan and Meixiangzhan	4 to 5 months	At least 10 PCR-positive T0 plants
	Single-gene knockout			At least 5 T0 edited lines
	Multi-gene knockout			At least 5 T0 edited lines
Forage grasses (Bermudagrass, Kentucky bluegrass, Creeping bentgrass, Zoysiagrass)	Genetic transformation	Baron,Common,Tiger, Companion	6 to 8 months	At least 5 T0 plants
	Single-gene knockout			At least 5 T0 edited lines
	Multi-gene knockout			At least 5 T0 edited lines
Soybean	Genetic transformation	Soybean varieties such as W82, Jack, Zhonghuang 302, and Jiyu 86	4 to 5 months	At least 15 positive T0 plants
	Single-gene knockout		5 to 6 months	At least 5 T0 edited lines
	Multi-gene knockout		5 to 6 months	At least 5 T0 edited lines
	Precision Editing (PE)		7 to 8 months	At least 3 T0 edited lines
Maize	Genetic transformation	Research maize varieties such as B104, KN5585, and B73	8 to 10 months	At least 10 batches of T1 generation Bar test strip–positive seeds
	Single-gene knockout		8 to 10 months	At least 5 batches of T1 generation edited seeds
	Multi-gene knockout		8 to 10 months	At least 5 batches of T1 generation edited seeds
	Precision Editing (PE)		10 to 12 months	At least 3 T0 generation edited lines
	Genetic transformation	Core inbred lines such as Z58, C7-2, Ph6wc, and Ph4cv	8 to 10 months	At least 10 batches of T1 generation Bar test strip–positive seeds
	Single-gene knockout		8 to 10 months	At least 5 batches of T1 generation edited seeds
	Multi-gene knockout		8 to 10 months	At least 5 batches of edited T1 generation seeds
	Precision Editing (PE)		10 to 12 months	At least 3 edited T0 lines
Tomato	Genetic transformation	AC, MicroTom, Monymaker, and other tomato varieties	4 to 5 months	At least 15 T0 generation positive plants
	Single-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Multi-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Precision Editing (PE)		5 to 6 months	At least 3 edited T0 lines
Lettuce	Genetic transformation	Green Butterfly, Rosa Verde, Da Su Sheng, and other lettuce varieties	4 to 5 months	At least 15 positive T0 plants
	Single-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Multi-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Precision Editing (PE)		5 to 6 months	At least 3 edited T0 lines
Tobacco	Genetic transformation	Nicotiana benthamiana	5 to 6 months	At least 15 positive T0 plants
	Single-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Multi-gene knockout		5 to 6 months	At least 5 edited T0 lines
	Precision Editing (PE)		5 to 6 months	At least 3 edited T0 lines
Arabidopsis thaliana	Genetic transformation	Col-0	5 to 6 months	At least 15 positive T1 lines
	Single-gene knockout		6 to 7 months	At least 5 edited T1 lines
	Multi-gene knockout		6 to 7 months	At least 5 edited T1 lines
	Precision Editing (PE)		6 to 7 months	At least 3 edited T1 lines
Solanum tuberosum	overexpression	Desiree	4 to 6 months	At least 5 T0 transgenic lines with positive seedlings
Solanum tuberosum	single-gene knockout	Desiree	4 to 6 months	At least 3 independent T0 genome-edited lines

Sample Submission Requirements

Types	Sample submission standards	Transport conditions
The sequence needs to be synthesized	None	None
Constructed plasmid Intermediate vector plasmid	Concentration >100 ng/μl, volume >20 μl; vector sequence and antibiotic resistance information provided; no contamination	Ice pack
Bacterial culture containing the constructed plasmid Bacterial culture containing the intermediate vector plasmid	Volume >500 μl; vector sequence and antibiotic resistance information provided; no contamination	Dry ice: 5 kg for 1 day, 10–20 kg for 2–3 days, 20–30 kg for 3–5 days.
Bacterial colonies on agar plate	No contamination	Room temperature
Seeds (uncommon variety)	200+ seeds	Room temperature

Case Result

Disclaimer: All products and services are intended for research use only. Not for diagnostic or therapeutic applications.

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

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