Our service
To provide biological high-end technical services for scientific workers in the field of basic research
Two-Hybrid (Membrane System)
Technology
DUAL membrane system designed to identify interactions involving integral membrane proteins or membrane-associated proteins is derived from Dualsystems Biotech AG. The quality Two-Hybrid Membrane System discovers and studies the functions and interaction networks of membrane proteins by detecting how protein fusions bind to membrane proteins and activate transcription.
Designed to detect novel interaction partners for membrane proteins, the DUAL membrane System can be used to detect pairwise protein interactions, identify novel protein interactions by cDNA library screening, investigate ternary complexes, and map interaction domains. Scientific studies involved include membrane protein-membrane protein or membrane protein-soluble protein interaction, screening of expression library, and verification of the interaction relationship between two known proteins (one of them is a membrane protein).
Service process
Service content |
Working days |
1. Gene synthesis(Codon Optimization) /Vector Construction |
15d |
2. Self-activation of bait plasmid |
10 d |
3. Co-transfer Bait plasmid and library plasmid 4. Selective medium for yeast screening 5. Sanger sequence of screening results |
15 d
|
6. Verify by Rotation 7. Temporary Data Report |
5 d |
8. Next-generation sequencing 9. Complete Project Data Report |
10 d |
Total |
55 d |
Features
1. Research Proteins located on cell membrane, or other organelles’ membrane, or membrane-associated receptors;
2. Based on NGS, delivery more positive clone sequences
3. Ensure at least 8 positive clone sequences (No Frameshift Mutation);
4. ProS-3D Structural Analysis
5. Recommended Whole Sequencing Library
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Tel: +86 025-85205672
Email: info@pronetbio.com
Room 1001, Floor 10, Building 3C, Nanjing Xianlin Zhigu, No. 6 Qimin West Road, Xianlin Street, Qixia District, NanJing, Jiangsu, China, 210033
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