Our service

To provide biological high-end technical services for scientific workers in the field of basic research

Conventional Yeast Platform

Nanobody Platform

AI Screening Protein-Interactins

Two-Hybrid (Membrane System)

Technology

DUAL membrane system designed to identify interactions involving integral membrane proteins or membrane-associated proteins is derived from Dualsystems Biotech AG.

Designed to detect novel interaction partners for membrane proteins, the DUAL membrane System can be used to detect pairwise protein interactions, identify novel protein interactions by cDNA library screening, investigate ternary complexes, and map interaction domains. Scientific studies involved include membrane protein-membrane protein or membrane protein-soluble protein interaction, screening of expression library, and verification of the interaction relationship between two known proteins (one of them is a membrane protein).

Two-Hybrid (Membrane System)

Service process

Service content	Working days
1. Gene synthesis(Codon Optimization) /Vector Construction	15d
2. Self-activation of bait plasmid	10 d
3. Co-transfer Bait plasmid and library plasmid 4. Selective medium for yeast screening 5. Sanger sequence of screening results	15 d
6. Verify by Rotation 7. Temporary Data Report	5 d
8. Next-generation sequencing 9. Complete Project Data Report	10 d
Total	55 d

Features

1. Research Proteins located on cell membrane, or other organelles’ membrane, or membrane-associated receptors；

2. Based on NGS, delivery more positive clone sequences

3. Ensure at least 8 positive clone sequences (No Frameshift Mutation)；

4. ProS-3D Structural Analysis

5. Recommended Whole Sequencing Library

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!