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Yeast Secreted Protein Expression Vector Kit
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Discover our Yeast Secreted Protein Expression Vector Kit, utilizing the Pichia pastoris system with AOX1 promoter for high-yield recombinant protein production. Includes pPICZαA and pPIC9K vectors, GS115 and X33 strains, and AOX primers for efficient amplification and sequencing.
INSTRUCTION & COA：
- Product Description
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Product Description
The Yeast Secreted Protein Expression Vector Kit provides a complete solution for efficient protein expression using the mature Pichia pastoris expression system. This system utilizes the alcohol oxidase 1 (AOX1) promoter to initiate protein production, enabling growth on methanol as the sole carbon source. The kit includes two commonly used expression vectors, pPICZαA and pPIC9K, along with two strains: GS115 (his4) and X33 (wild type). The pPICZαA vector is designed for expression with the X33 strain, while the pPIC9K vector pairs with the GS115 strain. Primers 5’AOX and 3’AOX are provided for amplification and sequencing purposes.
CAT: RY8014
Price and Instruction: Available upon inquiry
Key Features
High Protein Yield: Achieve elevated levels of protein expression due to the strong AOX1 promoter.
Eukaryotic Processing: Benefit from proper protein folding and post-translational modifications inherent to eukaryotic systems.
Scalability: Easily scale from small laboratory setups to large industrial fermentations.
Versatility: Suitable for both intracellular and secreted protein expression applications.
Applications
This kit is ideal for researchers aiming to express recombinant proteins in a eukaryotic system, particularly when post-translational modifications are essential. It is suitable for producing enzymes, antibodies, and other proteins for research and industrial applications.
Product Components
Component
Name
Concentration
Specification
Quantity
Plasmid
pPIC9K
100 ng/μL
20 μL
1 tube
Plasmid
pPICZαA
100 ng/μL
20 μL
1 tube
Plasmid
Positive Control Plasmid
100 ng/μL
20 μL
1 tube
Strain
GS115
Inoculated Plate
1 plate
1 plate
Strain
X-33
Inoculated Plate
1 plate
1 plate
Primer
5’AOX
Lyophilized
2 OD
1 tube
Primer
3’AOX
Lyophilized
2 OD
1 tube
Storage and Shipping Conditions
Plasmids: Store at -20°C; shipped at room temperature.
Strains: Store at room temperature; shipped at room temperature.
Primers: Store at -20°C; shipped at room temperature.
Experimental Guidelines
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
Why Choose This Kit?
Ease of Use: Culturing Pichia pastoris is as straightforward as E. coli, making it accessible for various laboratory settings.
High Cell Density: Achieve higher cell densities compared to other microbial expression systems, leading to increased protein yield.
Eukaryotic Protein Processing: Benefit from eukaryotic mechanisms for protein folding and post-translational modifications, resulting in functionally active proteins.
Cost-Effective Scaling: The ability to grow on methanol as a sole carbon source makes scaling up to industrial levels relatively inexpensive.
Ready to Advance Your Research?
Contact us today for a detailed consultation and product quotation. Let us help you unlock new insights in your research. When inquiring about this product, please reference Product Code: RY8014.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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