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Yeast Secreted Protein Expression Vector Kit
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One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts, molds, mammals, plants, and insects.
INSTRUCTION & COA：
- Product Description
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Catalog Number:
RY8014
Storage:
Transport at Room Temperature.
After the primers are dissolved and stored at 4°C.
It is recommended to activate the strain on YPDA medium immediately upon receipt.
For long-term storage, it is recommended to prepare a glycerol stock (in 15% glycerol) for the activated strain and store at –80°C.
Product Description
One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts, molds, mammals, plants, and insects. Yeast expression systems such as Saccharomyces cerevisiae (S. cerevisiae) and Pichia pastoris (P. pastoris) are more popular. P. pastoris expression system is one of the most popular and standard tools for the production of recombinant protein in molecular biology. Overall, the benefits of protein production by P. pastoris system include appropriate folding (in the endoplasmic reticulum) and secretion (by Kex2 as signal peptidase) of recombinant proteins to the external environment of the cell. Moreover, in the P. pastoris expression system due to its limited production of endogenous secretory proteins, the purification of recombinant protein is easy. It is also considered a unique host for the expression of subunit vaccines which could significantly affect the growing market of medical biotechnology.
Our Yeast Secreted Protein Expression system is based on Pichia pastoris.
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins, 2019
Components
Components
Forms
Size
GS115 Strain
Solid culture medium
One plate (diameter: 15cm)
X-33 Strain
Solid culture medium
One plate (diameter: 15cm)
pPIC9K Plasmid
Liquid
2 ug（20ul,100ng/ul)
pPICZαA Plasmid
Liquid
2 ug（20ul,100ng/ul)
Positive Control Plasmid
Liquid
2 ug（20ul,100ng/ul)
5’AOX 1
Lyophilized powder
2OD
3’AOX 1
Lyophilized powder
2OD
Note: The positive control protein is about 27kD.
Experiment Process
Related products:
Yeast Colony Rapid Detection Kit Catalog No.: RY8001
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Email: info@pronetbio.com
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