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Soybeans Genetic Transformation

Project Brief

The explants of soybeans (such as cotyledon nodes, germ tips, or mature hypocotyl segments) are co-cultured with Agrobacterium. During the co-culture process, the Agrobacterium adheres to the surface of the soybean explant cells and transfers into the soybean cells. The target gene carried by T-DNA will randomly integrate into the genome of the soybean cells. Finally, by utilizing the totipotency of plant cells, the transformed soybean cells are cultivated into complete transgenic plants through tissue culture techniques.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service process, Cycle and Delivery

Species	Type of service	Variety	Service Period	Delivery Standard (Default delivery includes resistance gene identification)
Soybean	1. Vector construction / Gene synthesis 2. Sequencing of the target gene + Vector construction 3. The target gene has been constructed into an expression vector and its sequence has been verified through sequencing.	Williams 82, Dongnong 50, Hainong 81	ten	1. Vector Construction: The client does not provide the vector template and does not deliver it. 2. Gene Synthesis: The client does not provide the vector template and does not deliver it.
	overexpression		4 to 6 months	The number of T0 generation positive plants (resistant plants) is ≥ 10.
	overexpression		9 to 12 months	Five T0 positive seedlings were harvested to obtain T1 seeds (a total of 5 samples, the exact weights are subject to actual measurement)
	Single-gene knockout		4 to 6 months	The number of T0 generation positive seedlings (resistant seedlings) is ≥ 3.
	Single-gene knockout		9 to 12 months	Three T0 positive seedlings were harvested to obtain T1 seeds (a total of 3 samples, the exact weights are subject to actual measurement)

Note: Other varieties can be tested*.

Case Show

A: Disinfection B: Absorption and swelling C: Infection D: Co-culture E: Screening F: Differentiation G: Rooting H: Conditioning of seedlings

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