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Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

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Interaction Validation Platform

Rice Genetic Transformation

Project Brief

The explants (callus) of rice were co-cultured with Agrobacterium. During the co-culture process, the Agrobacterium would attach to the surface of the rice explant cells and transfer the T-DNA into the rice cells. The target gene carried by the T-DNA would randomly integrate into the genome of the rice cells. Finally, by utilizing the totipotency of plant cells and through tissue culture techniques, the transformed rice cells were cultivated into complete transgenic plants.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	service period	Delivery Standard (Default delivery includes resistance gene identification)
rice	1. Vector construction / gene synthesis 2. Target gene sequencing + vector construction 3. The target gene has been cloned into an expression vector, with sequencing verification of the target gene	/	ten	1. Vector construction: If the vector template is not provided by the client, it will not be delivered. 2. Gene synthesis: If the vector template is not provided by the client, it will not be delivered.
japonica rice	overexpression	Japanese varieties such as Kyouei, Chōkawa 11, and Kitake, which are all indica rice varieties	2.5 to 3 months	The number of positive strains in the T0 generation was ≥ 15.
	overexpression		7.5 - 9 months	10 T0 positive seedlings were harvested to obtain T1 seeds (a total of 10 samples, the exact weights are subject to actual measurement)
	Single-gene knockout		2.5 to 3 months	The number of positive strains in the T0 generation was ≥ 10.
	Single-gene knockout		7.5 - 9 months	Five T0 positive seedlings were harvested to obtain T1 seeds (a total of 5 samples, the exact weights are subject to actual measurement)
nonglutinous rice	overexpression	The indica rice varieties such as Huang Huazhan and Mei Xiangzhan	4 to 5 months	The number of T0 generation positive plants (resistant plants) is ≥ 10.
	overexpression		9 to 11 months	Five T0 positive seedlings were harvested to obtain T1 seeds (a total of 5 samples, the exact weights are subject to actual measurement)
	Single-gene knockout		4 to 5 months	The number of T0 generation positive plants (resistant plants) is ≥ 5.
	Single-gene knockout		9 to 11 months	Three T0 positive seedlings were harvested to obtain T1 seeds (a total of 3 samples, the exact weights are subject to actual measurement)