Services
Providing Advanced Biological Technical Services for Researchers in Fundamental Science
Rice Genetic Transformation Service
From Gene to Seedling: Comprehensive Rice Transformation Solutions
Overview
Our Rice Genetic Transformation Service utilizes Agrobacterium tumefaciens-mediated gene delivery to introduce foreign genes into rice callus tissues. During co-cultivation, Agrobacterium attaches to the surface of rice explants and transfers T-DNA containing the gene of interest into plant cells, where it integrates randomly into the rice genome. With the totipotency of plant cells, transformed calli are regenerated into transgenic rice plants through optimized tissue culture techniques.
Service Process
Sample and vector information confirmation → Experimental design → Callus induction → Transformation and infection → Selection and rooting → PCR and sequencing analysis → Transgenic seedling delivery
Key Advantages
High Screening Efficiency: Our optimized media achieve over 60% positive callus rates.
End-to-End Monitoring: Each step, from seed sterilization to regeneration, is strictly quality controlled.
qPCR Validation: In addition to conventional PCR, we provide fluorescence quantitative PCR for enhanced gene function verification.
Flexible Selection Markers: Supports selection via hygromycin, phosphinothricin (Basta), and glyphosate resistance.
Service Specifications
Vector Type | Application | Timeline | Deliverables |
|---|---|---|---|
Standard Transformation Vector | Overexpression / RNAi | 10–15 working days | Plasmid construction + Sequencing report |
CRISPR/Cas9 Editing Vector | Gene Knockout / Editing | Same as above |
Rice Varieties and Transformation Timeline
Rice Type | Cultivars | Duration | Deliverables |
|---|---|---|---|
Japonica | Nipponbare, Kitaake, Xiushui, Nanjing, Wuyunjing series | 2.5–3 months | 20 T0 seedlings, PCR + Sanger sequencing + qPCR reports |
Indica | 9311, Kasalath, Huazhan, Wushan Simiao, Zhongjiazao 17 | 4–5 months | 5 T0 seedlings, PCR + Sanger sequencing + qPCR reports |
Sample Submission Requirements
Type | Requirements | Shipping Conditions |
|---|---|---|
Synthesized Gene | — | — |
Purified Plasmid | >100 ng/µL, >20 µL, resistance info provided, no contamination | Ice pack |
Bacterial Culture (Plasmid) | >500 µL, resistance info provided, no contamination | Dry ice (1–5 days depending on weight) |
Plate Culture | No contamination | Room temperature |
Seeds (rare cultivars) | >200 seeds | Room temperature |
Timeline & Deliverables
Stage | Duration | Deliverables |
|---|---|---|
Vector Construction | 10–15 working days | Verified vector plasmid |
Transformation | ~2 months | T0 positive seedlings |
PCR & Sequencing | 5 working days | PCR report, Sanger sequencing |
qPCR Analysis (Optional) | 10 working days | Raw data, fluorescence images, experimental report |
Total Time | ~3.5 months | Comprehensive data package |
Service Case Results
Note: All products and services are strictly intended for research use only. They are not to be used for clinical diagnostics or therapeutic applications.
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Product Inquiry
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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