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Salvia Miltiorrhiza Genetic Transformation

Project Brief

Salvia miltiorrhiza is an important medicinal plant whose active compounds, such as tanshinones and salvianolic acids, exhibit significant cardiovascular protective effects. This project aims to establish an efficient genetic transformation system for Salvia miltiorrhiza by introducing foreign genes (such as reporter genes like GUS or functional genes) into its genome via Agrobacterium-mediated transformation, thereby providing a technical foundation for subsequent gene function studies and molecular breeding of desirable traits.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	Service Period	Delivery Standard(Default delivery of resistance gene identification)
Salvia miltiorrhiza	1. Vector construction / Gene synthesis 2. Sequencing of the target gene + Vector construction 3. The target gene has been constructed into an expression vector and its sequence has been verified through sequencing.	Hebei Salvia miltiorrhiza	ten	1. Vector Construction: The client does not provide the vector template and does not deliver it. 2. Gene Synthesis: The client does not provide the vector template and does not deliver it.
	overexpression		6 to 8 months	The number of positive strains in the T0 generation was ≥ 3.
	Single-gene knockout		6 to 8 months	The number of positive strains in the T0 generation was ≥ 1.

Note: Other varieties can be tested*.

Case Show

From left to right: pre-culture, co-culture, callus induction, shoot induction, and root induction

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