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Dual-Luciferase Reporter Gene Kit
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Sensitive dual-luciferase reporter gene kit for promoter activity and gene expression analysis in plants. Includes pGreenII 0800-LUC and pBinGFP2 plasmids.
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- Product Description
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Dual-Luciferase Reporter Gene Kit
Catalog No.: RY8020
Application: Gene Expression Regulation Analysis | Plant Molecular Biology | Promoter Activity DetectionOverview
The Dual-Luciferase Reporter Gene Kit offers a reliable and sensitive system for detecting changes in gene expression and analyzing transcriptional regulation in plant cells. Based on a bioluminescent reaction between luciferase enzymes and their specific substrates, this kit enables real-time monitoring of promoter activity or gene regulation by quantifying emitted luminescence.
This kit includes two widely used reporter plasmids: pBinGFP2 and pGreenII 0800-LUC, as well as validated primer pairs for PCR amplification or sequencing. It is ideal for plant transient expression assays and dual-luciferase activity comparisons.
Key Features
Sensitive and Quantitative: Enables accurate detection of gene expression changes through dual-luciferase comparison.
Dual-Plasmid System: Combines firefly luciferase and Renilla luciferase to reduce variability and improve data normalization.
Plant-Optimized: Designed for use in Nicotiana benthamiana (tobacco) and compatible with Agrobacterium-mediated transformation.
Ready-to-Use: Includes all required plasmids and primers for rapid experimental setup.
Kit Components
Component
Specification
Antibiotic Resistance Shipping Temp. Storage Temp. pBinGFP2 Plasmid
2 µg (20 µL at 100 ng/µL)
Kanamycin (Kan) RT 4°C (short-term), –20°C (long-term) pGreenII 0800-LUC Plasmid
2 µg (20 µL at 100 ng/µL)
Kanamycin (Kan) positiveA-pBinGFP2 Plasmid
2 µg (20 µL at 100 ng/µL)
Kanamycin (Kan) pBinGFP2-F Primer
Lyophilized, 2 OD
— pBinGFP2-R Primer
Lyophilized, 2 OD
— pLUC-F Primer
Lyophilized, 2 OD
— pLUC-R Primer
Lyophilized, 2 OD
— Note: All plasmids are shipped at room temperature (RT) and should be stored at –20°C for long-term use. Lyophilized primers should be centrifuged and reconstituted with nuclease-free water before use.
Required Materials (Not Included)
Biological Materials:
Nicotiana benthamiana plants
Reagents:
E. coli TOP10 competent cells
Agrobacterium tumefaciens GV3101 competent cells
Restriction enzymes, ligases, recombinase
Plant buffers and media: LB, TB buffer, MgCl₂, DMSO, lysis buffer, etc.
Consumables:
Syringes, pipette tips, centrifuge tubes, PCR tubes, 96-well microplates, disposable gloves, etc.
Equipment:
Incubator, PCR machine, plate reader, electrophoresis system, ChemiDoc or similar imaging system, centrifuge, microwave, electroporator, -80°C freezer, etc.
Applications
· Promoter activity analysis;
· Transcriptional regulation studies;
· Gene expression quantification;
· High-throughput screening for regulatory elements in plants;
Usage Notes
1. Upon receipt, plasmids should be centrifuged briefly and transformed into E. coli for amplification.
2. This product is intended for research use only. It is not suitable for human, animal, or food use.
3. Always wear a lab coat and gloves when handling the product to ensure safety and sample integrity.
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Explore how the Dual-Luciferase Reporter Gene Kit can accelerate your plant molecular biology research today.Related Service: Dual Luciferase Reporter Gene Technology Service
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Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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