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Yeast Signal Peptide Detection Vector Kit
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Explore the Yeast Signal Peptide Detection Vector Kit for accurate and efficient screening of signal peptide secretion functions in yeast expression systems. Featuring high sensitivity and easy-to-use TTC colorimetric reactions, this kit ensures reliable results for protein secretion studies. Perfect for optimizing recombinant protein production.
INSTRUCTION & COA：
- Product Description
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Yeast Signal Peptide Detection Vector Kit – Reliable Screening for Signal Peptide Secretion Function
Product Overview
The Yeast Signal Peptide Detection Vector Kit is a highly effective tool designed for screening and verifying the secretion function of signal peptides in yeast expression systems. This kit uses the YTK12 yeast strain, which lacks the invertase gene and cannot grow on media where sucrose is the sole carbon source. The included pSUC2 plasmid contains a tryptophan synthesis gene and an invertase gene (SUC2) missing its signal peptide and the start codon (ATG). When a functional signal peptide is fused to SUC2, the invertase secretion function is restored, allowing the YTK12 strain to grow on selective media with raffinose as the carbon source.
To verify the signal peptide secretion functionality, a TTC (triphenyltetrazolium chloride) colorimetric reaction is used. This simple and reliable method allows researchers to confirm the success of their signal peptide fusions and optimize their yeast expression systems for downstream applications.
Ideal for use in protein secretion studies, this kit provides a straightforward and effective solution for researchers working with yeast as a model organism.
CAT：RY8015
Price and Instruction: Available upon inquiryProduct Components
Component
Name
Concentration
Specification
Quantity
Plasmids
pSUC2
100ng/uL
20uL
1 tube
pSUC2-Avr1b
100ng/uL
20uL
1 tube
Strain
YTK12
Inoculated Plate
Diameter:90mm
1 plate
Primers
pSUC2-F
Lyophilized powder
2OD
1 tube
pSUC2-R
Lyophilized powder
2OD
1 tube
Experimental Guidelines
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
Storage and Shipping Conditions
Plasmids: Store at -20°C; shipped at room temperature.
Strains: Store and ship at room temperature.
Primers: Store at -20°C; shipped at room temperature.
Product Advantages
High Sensitivity: The kit enables precise identification and verification of signal peptide secretion functions.
Ease of Use: Simple protocol with TTC colorimetric reaction for easy verification.
Versatile: Suitable for various yeast expression systems and signal peptide research applications.
Reliable Results: The combination of YTK12 strain and pSUC2 plasmid ensures consistent and reproducible results.
FAQs
Q: What is the main application of the Yeast Signal Peptide Detection Vector Kit?
A: The Yeast Signal Peptide Detection Vector Kit is used to screen and verify the secretion function of signal peptides in yeast expression systems. It is essential for protein secretion studies and optimizing recombinant protein production.Q: Can this kit be used with other yeast strains?
A: While the kit is optimized for the YTK12 strain, it can potentially be adapted to other yeast strains, depending on their genetic characteristics. Please consult the product documentation for more details.Q: How do I store the yeast strain and plasmid after receipt?
A: The yeast strain should be stored at room temperature and preserved in glycerol at -80°C. The plasmids should be stored at -20°C. For more detailed storage instructions, refer to the product specifications.Contact Us Today
Unlock the full potential of your yeast expression systems with the Yeast Signal Peptide Detection Vector Kit! Whether you're optimizing protein secretion or verifying signal peptide functionality, this kit provides you with precise, reliable results. Contact us below to learn more about how this kit can enhance your research or to request pricing and detailed instructions. Let us help you streamline your studies for more efficient and accurate protein production!
Disclaimer: This product is intended for research purposes only. It should not be used for any clinical, diagnostic, or food-related applications.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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