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Providing Advanced Biological Technical Services for Researchers in Fundamental Science
Yeast Three-Hybrid (Y3H) Screening Service
Yeast Three-Hybrid (Y3H) Screening Service for Advanced Protein Interaction Analysis
The Yeast Three-Hybrid (Y3H) system is a next-generation platform built on the Yeast Two-Hybrid (Y2H) technology. It provides a unique solution for detecting three-way molecular interactions, making it ideal for studying protein-protein-small molecule binding, protein-DNA or protein-RNA interactions, and other complex biological processes.
Utilizing an innovative pBridge plasmid, this system enables precise protein expression under methionine-deficient conditions, ensuring robust and reproducible results. Backed by advanced screening and validation workflows, our Y3H service (Service Code: SRY8011) supports groundbreaking discoveries in drug development, functional genomics, and signal pathway analysis.
Why Choose Yeast Three-Hybrid Screening with Us?
Comprehensive Interaction Analysis: Study interactions between three molecular partners, including proteins, nucleic acids, and small molecules.
Efficient Screening: Accelerated workflows with detailed results for high-throughput experiments.
Tailored to Your Research: Custom library options and optimized screening conditions for specific needs.
Reliable Results: Robust protocols with validated positive clones and in-depth analysis reports.
Applications
Drug Discovery: Identify modulators affecting protein-protein or protein-small molecule interactions.
Functional Genomics: Investigate complex nucleic acid-binding protein mechanisms.
Signal Pathway Studies: Analyze intricate signaling cascades involving multiple interaction partners.
Technical Advantages
High Precision: Advanced pBridge plasmid technology ensures in vivo validation under methionine-deficient conditions.
Wide Applicability: Supports protein complexes, post-translational modifications, and nucleic acid-binding studies.
Streamlined Processes: Optimized protocols minimize turnaround time while maximizing accuracy.
Comprehensive Deliverables: Includes positive clone sequences, detailed project reports, and sequencing data.
Service Workflow: Yeast Three-Hybrid Library Screening
Step | Description | Duration |
|---|---|---|
1. Gene Synthesis & Vector Construction | Codon optimization, synthesis of target genes, and construction of pBridge plasmids. | 15 days |
2. Self-Activation Testing | Detection and optimization of bait plasmid self-activation in selective media. | 10 days |
3. Co-Transformation and Screening | Co-transform bait plasmids with cDNA library and screen in quintuple-deficient media. | 20 days |
4. Reverse Validation | Re-validation of positive interactions using additional controls and media. | 5 days |
5. NGS Sequencing & Analysis | Next-generation sequencing, data collation, and interaction network analysis. | 10 days |
Example Workflow: How It Works
Plasmid Preparation: Construct pBridge-X-Y plasmid, incorporating two proteins of interest under methionine-dependent expression.
Transformation: Introduce pBridge-X-Y into Y2HGold yeast and pGADT7 into AH109 yeast.
Co-Culture and Screening: Co-culture both strains and screen on methionine-deficient selective media (SD/-Met/-Ade/-His/-Leu/-Trp).
Positive Clone Validation: Validate positive interactions through Sanger or NGS sequencing and functional assays.
Deliverables
Positive Clones: Sequencing data for ≥15 positive clones (Based on Sanger sequencing).
Reports: Comprehensive project reports in Word/PDF formats.
Plasmids: Ready-to-use bait plasmids (optional).
Screening Results: Comprehensive interaction data and reproducibility workflow.
Frequently Asked Questions (FAQ)
1. What is the minimum starting material required?
We recommend a prepared library or gene of interest for cloning into pBridge or pGADT7 vectors.
2. Can this service analyze nucleic acid interactions?
Yes, the Y3H system excels in studying protein-DNA and protein-RNA interactions under in vivo conditions.
3. How long does the entire process take?
Typical projects are completed within 50–60 working days, depending on complexity and library size.
Get Started with Y3H Screening
Ready to accelerate your research with Yeast Three-Hybrid technology?
Contact our experts today for a personalized consultation, experiment protocol or request a quote now to begin your project!
When inquiring about this service, please reference Service Code: SRY8011.
Disclaimer: Our services are sold for research purposes only and are not intended for diagnostic or therapeutic use!
Related Product: Yeast Three-Hybrid (Nuclear System) Vector Kit [CAT：RY8011]
Related Services: Explore our EMSA and Pull-Down Assays for advanced molecular interaction validation.
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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