Our service

To provide biological high-end technical services for scientific workers in the field of basic research

Conventional Yeast Platform

Nanobody Platform

AI Screening Protein-Interactins

Conventional Molecular Biology Platform

Nanobody Screening (Nuclear System)

Technology

The customized Nanobody Screening Nuclear System can quickly and efficiently screen and identify nanobodies that function in the nucleus, helping to deeply understand the functions and interaction mechanisms of nuclear proteins, thereby promoting research progress in related fields.

The antigen-binding capacity of nanobodies is similar to conventional antibodies for three reasons. First, the complementarity-determining region 3 (CDR3) of nanobodies is similar to or even longer than that of the human VH domain (variable domain of heavy immunoglobulin chain). Second, nanobodies can form finger-like structures to recognize cavities or hidden epitopes that are not available to mAbs. Third, nanobodies exhibit excellent stability, hydrophilicity, and water solubility that help maintain their binding affinity across different conditions.

Nanobodies can be used for early and rapid diagnosis of disease, which is essential for detecting some highly pathogenic, infectious and potentially deadly pathogens.

Nanobody Screening (Nuclear System)

Service process

Service content	Working days
1. Gene synthesis(Codon Optimization)/Vector Construction	15 d
2. Self-activation of bait plasmid	10 d
3. Co-transfer Bait plasmid and VHH library plasmid 4. Selective medium for yeast screening 5. Sanger sequence of screening results	20 d
6. Verify by rotation 7. Complete Project Data Report	5 d
Total	50 d

Features

1. Synthetic Library, No Species Limited

10^8 = 100 million fragments, and No Frameshift Mutation

2. Ensure at least 5 CDR3 positive clone sequences

3. Comparable accuracy but Lower Cost (compared with phage library screening)

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!