Our service
To provide biological high-end technical services for scientific workers in the field of basic research
Abiotic-Stress Resistance Gene Screening
Technology
The advantage of Saccharomyces cerevisiae Surface Display is that it can directly display proteins, antibodies, and other molecules on the surface of living cells, which facilitates real-time observation and screening; Saccharomyces cerevisiae belongs to eukaryotes, which is closer to the expression system of plants and animals. This expression system includes the following processes: glycosylation, disulfide bond formation, and Post-translational modification of protein folding. Therefore, the protein encoded by the plant gene screened out by the yeast system can function normally, and the false positive probability of multiple resistance genes is greatly reduced. The resistance-related genes were screened from the whole genome through the resistance gradient experiment, and the screening results were analyzed by bioinformatics to classify the resistance genes.
Service process
Service content |
Working days |
|
Yeast working solution preparation |
1. Yeast working solution preparation. 2. PCR quality control for working solution. |
10 d |
Stress condition optimization |
3. Five different concentration gradient mediums for condition optimization. |
10 d |
Yeast Screening |
4. yeast suspension coating on 20 plates (diameter: 15cm). |
10 d |
Positive clone identification |
5. PCR identification of yeast colonies 6. Sanger sequence of screening results |
5d |
Verify the Screening results |
7. Verify by Rotation 8. Temporary Data Report |
10 d |
NGS |
9. Next-generation sequencing 10. Complete Project Data Report |
15 d |
Total |
|
60 d |
Features
1. Recommended Species
2. Screen single or multiple genes
3. Combined with next generation sequencing
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
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Tel: +86 025-85205672
Email: info@pronetbio.com
Room 1001, Floor 10, Building 3C, Nanjing Xianlin Zhigu, No. 6 Qimin West Road, Xianlin Street, Qixia District, NanJing, Jiangsu, China, 210033
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