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Second-Generation Yeast Colony PCR Kit
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Discover ProNet Biotech’s Second-Generation Yeast Colony PCR Kit—engineered for fast lysis, robust plasmid amplification, and direct gel loading. Compatible with all yeast strains and ideal for TA cloning. Boost your lab efficiency today.
INSTRUCTION & COA：
- Product Description
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High-Performance Yeast Colony PCR Kit for Rapid and Reliable Amplification
ProNet Biotech's Second-Generation Yeast Colony PCR Kit offers a fast, reliable, and high-throughput solution for amplifying target sequences from yeast colonies. Powered by our enhanced Yeast Buster Pro lysis buffer and green-dye-loaded PCR mix, this kit ensures superior lysis efficiency, high product stability, and excellent compatibility with downstream applications like electrophoresis and TA cloning.
Product Highlights
· Rapid Lysis with Yeast Buster Pro: Efficiently breaks yeast cell walls and preserves DNA integrity.
· Ready-to-Load PCR Mix: Includes green dye for direct gel electrophoresis post-amplification.
· Freeze-Thaw Stable: Contains stabilizers to ensure performance consistency.
· TA-Cloning Ready: PCR products have 3' A-overhangs, suitable for TA cloning.
· Compatible with All Yeast Strains: Optimized for Saccharomyces cerevisiae and other yeast types.
Application Scope
· Suitable for all types of yeast strains
· Designed for rapid amplification of recombinant plasmid target sequences directly from yeast colonies
Kit Components
Component
40T
80T
160T
320T
2× Yeast PCR Mix
1 ml
1 ml × 2
1 ml × 4
1 ml × 8
Yeast Buster Pro
200 μl
400 μl
800 μl
1.6 ml
Workflow Overview
From Colony to Gel in 3 Simple Steps
· Yeast Cell Lysis: Use Yeast Buster Pro to disrupt cells at 100°C for 5 min.
· PCR Reaction: Amplify target gene with optimized primers and ready-to-use 2× PCR mix.
· Gel Electrophoresis: Load PCR product directly onto gel for analysis.
Experimental Protocol Summary
Lysis Reaction Setup:
Add 5 μl Yeast Buster Pro to PCR tube
Pick colony and suspend in lysis buffer
Heat at 100°C for 5 min
PCR Reaction Setup (Total volume: 50 μl):
25 μl 2× Yeast PCR Mix
2 μl Forward Primer (10 μM)
2 μl Reverse Primer (10 μM)
1 μl lysate as template
Add ddH₂O to 50 μl
Thermal Cycler Conditions:
Initial Denaturation: 95°C, 3 min
30–32 cycles of:
Denaturation: 95°C, 15 sec
Annealing: Tm − 5°C, 15 sec
Extension: 72°C, 15 sec/kb
Final Extension: 72°C, 5 min
Hold: 4°C
Common Troubleshooting Tips
Issue
Suggested Solution
Low/No Amplification
Increase cycles to 35–40; verify primer design and concentration
Smearing or Nonspecific Bands
Optimize annealing temp; reduce primer concentration
Weak Bands or Contaminants
Use high-purity template; extend PCR time
Ordering Information
Catalog Number: RY8031
Specifications: 40T / 80T / 160T / 320T
Storage: -20°C (avoid repeated freeze-thaw cycles)
Shelf Life: 12 months
Disclaimer: This product is intended solely for research purposes. It is not intended for human or veterinary diagnostic or therapeutic use.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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