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Interaction Validation Platform

Co-IP

Technical Background

Co-Immunoprecipitation (Co-IP) is a powerful and widely-used technique for studying protein-protein interactions based on the specific binding between antibodies and antigens. It enables the detection of protein interactions within living cells, providing crucial insights into the physiological roles of proteins and their impact on cellular functions and signaling pathways.

Co-IP offers several advantages over other protein interaction techniques such as yeast two-hybrid assays and GST pull-down assays. While these techniques are valuable, Co-IP allows for the isolation and identification of protein complexes in their natural state from complex cell lysates, minimizing false positives and avoiding artificial influences. This makes Co-IP an essential tool for studying protein interactions in their native cellular context.

Service Introduction

At ProNet Biotech, we offer a comprehensive suite of protein interaction validation services, including Co-IP, yeast two-hybrid assays, and GST pull-down assays. These services enable us to confirm protein interactions with high sensitivity and specificity.

Our Co-IP service utilizes transient expression in tobacco plants combined with the specific binding between antibodies and antigens to analyze protein interactions. This method offers a highly sensitive and widely applicable solution for studying protein interactions and can be seamlessly integrated with our yeast two-hybrid services for dual validation of protein interactions.

Whether you're looking to verify protein interactions identified through yeast two-hybrid assays or need a standalone protein interaction analysis, Nanjing ProNet Biotech provides reliable, high-quality services to support your research.

Technical Principle

Co-Immunoprecipitation (Co-IP) is based on the specific binding between antibodies and antigens, allowing for the isolation of protein complexes that interact within their native cellular environment. This technique leverages the natural binding interactions between proteins to provide highly accurate data about protein interactions, which is essential for understanding their roles in cellular processes and signaling.

Co-IP Suitable Applications

Protein Interaction Detection: Co-IP is ideal for determining whether two target proteins interact within living cells.
Protein Complex Isolation: This technique is suitable for isolating protein complexes that interact in their natural state, providing insights into the physiological roles of these complexes.

Sample Requirements

Sample Type	Sample Requirement	Preservation Condition	Transportation Condition
Plasmid Glycerobacterium	>500 µL in 1.5 or 2.0 centrifuge tubes	-20°C	Ice bag
Plasmid	>50 µg, packed in 1.5 or 2.0 centrifuge tubes	4°C	Ice bag
Antibody	Delivered according to antibody instructions, ensuring enough quantity for the experiment	-80°C	Dry ice
Sequence	Full sequence, no early termination	/	/

Service Content and Deliverables

Service Content	Period (Working Days)	Deliverables
Gene Optimization	15	1. Gene synthesis report 2. Sequencing verification report 3. E. coli DH5a/TOP10 with expression plasmids 4. Plant expression plasmid
Identification of Transient Expression in Plants	10	1. Expression analysis report 2. Plant cell lysate for Co-IP experiment
Co-IP Experiment	7	1. Original data and resulting images 2. Standard experimental report (including steps and procedures)

Case Studies

High Transformation Efficiency: We consistently achieve high transformation efficiency, ensuring reliable and reproducible results.

Authentic Data and High-Quality Images: Results include control groups, real data, and aesthetically pleasing images that clearly demonstrate protein interactions.

Protein Interaction Experimental Relationships

Co-IP is a primary method for detecting protein-protein interactions, but it should be viewed in relation to other experimental techniques like yeast two-hybrid assays and GST pull-down assays.

Yeast Two-Hybrid: This in vivo experiment helps verify potential protein interactions but can suffer from a higher false-positive rate. Therefore, further validation is often required.
Co-IP: Co-IP offers a lower false-positive rate and allows the analysis of protein interactions in their natural state. It is particularly effective for isolating naturally interacting protein complexes.
GST Pull-Down Assay: While Co-IP cannot determine whether the interaction is direct or indirect, the GST pull-down assay can be used to verify direct interactions by isolating the target protein in an in vitro setting.

Together, these methods offer a powerful combination for comprehensive protein interaction validation.

Why Choose ProNet Biotech?

Expertise: We specialize in protein interaction validation services and have a proven track record in providing high-quality data for our clients.

Comprehensive Services: We offer a full range of protein interaction analysis services, including yeast two-hybrid, Co-IP, and GST pull-down assays, providing integrated solutions for your research needs.

High Sensitivity: Our Co-IP service offers exceptional sensitivity and specificity for protein interaction analysis.

Get in Touch

Contact us today to discuss your project requirements and receive a customized solution. For more information or to request a quote, please contact us or fill out our information collection form to start your Co-IP validation project.

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