Our service

To provide biological high-end technical services for scientific workers in the field of basic research

Conventional Yeast Platform

Nanobody Platform

AI Screening Protein-Interactins

Conventional Molecular Biology Platform

Co-IP

Technical Background

Co-Immunoprecipitation (Co-IP) is a classic method based on the specific interaction between antibodies and antigens to study protein-protein interactions. It is used to determine the physiological interactions between two proteins within intact cells. Compared to other protein interaction techniques like yeast two-hybrid and pull-down assays, Co-IP has its advantages. By leveraging the binding between antigens and antibodies, Co-IP can specifically isolate and identify protein complexes from complex cell lysates, which is crucial for understanding how proteins interact within cells and how they influence cellular functions and signaling.

Service Introduction

Nanjing ProNet Biotech is a company specializing in protein interaction validation services, with an established yeast two-hybrid assay system. Building on this foundation, we are introducing a new protein interaction validation service—Co-IP. This technique uses transient expression in tobacco and the specific binding between antigens and antibodies to analyze protein interactions. It is highly sensitive and widely applicable, and can be used in conjunction with our yeast two-hybrid services for dual validation of protein interactions. Nanjing ProNet Biotech offers a full suite of protein interaction validation services, including yeast two-hybrid, Co-IP, and GST pull-down assays.

Technical Principle

Co-Immunoprecipitation (Co-IP) is a technique that analyzes protein interactions based on the specific binding between antibodies and antigens.

Suitable Applications for Co-IP

1. It can be used to detect whether two target proteins interact within a living cell.

2. It is suitable for isolating protein complexes that interact in their natural state.

Sample Requirements

Sample type	Sample requirement	Preservation condition	Transportation condition
Plasmid glycerobacterium	Each sample >500 ul in 1.5 or 2.0 centrifuge tubes	-20℃	Ice bag
Plasmid	Each sample >50 ug, packed with 1.5 or 2.0 centrifuge tubes	4℃	Ice bag
Antibody	Deliver according to the antibody instructions to avoid sub-packaging; If packaged separately, please ensure that there is enough for the experiment, and provide the antibody instructions. The amount of antibodies should be greater than the amount required for the experiment	-80℃	Dry ice
Sequence	Full sequence, no early termination	/	/

Service Content and Deliverables

Service content	Period(working days)	Delivery material
1.Gene optimization 1).Gene optimization and gene synthesis 2)Subclone into plant expression vector	15	1. Gene synthesis report 2. Sequencing verification report 3. E. coli DH5a/TOP10 containing expression plasmids 4. Plant expression plasmid
2.Identification of transient expression in plants 1). Agrobacterium transformation 2). Identification of positive clones 3). Transient expression of plants 4). Expression Analysis and Identification (WB)	10	1. Express analysis report 2. Plant cell lysate used in CO-IP experiment
3. Preparation of plant cell lysate 1). Protein extraction 2). Immunoprecipitation 3).Western Blot verification	7	1. Original data, resulting pictures 2. Standard experimental report (including experimental steps and procedures)

Case Studies

High transformation efficiency

Results include control groups, authentic data, and aesthetically pleasing images

Protein Interaction Related Experimental Relationships

The primary function of Co-IP is to detect protein interactions. There are many experimental methods to study protein interactions. Compared to widely used techniques like yeast two-hybrid and pull-down assays, what are the advantages and disadvantages of Co-IP, and what is their relationship?

Yeast two-hybrid is an in vivo experiment that can verify possible protein interactions, but due to its high false positive rate, further validation is needed.

At this point, Co-IP, with a relatively lower false positive rate, can play a role. Protein interactions can occur in their natural state, avoiding artificial influences, and allowing the isolation of naturally interacting protein complexes. Co-IP can further validate interactions based on yeast two-hybrid results. However, neither yeast two-hybrid nor Co-IP can prove whether the interaction is direct or indirect.

In such cases, the GST pull-down assay can be used. This in vitro experiment can remove the influence of other proteins, determining whether the target protein and the protein of interest can interact directly.

Nanjing ProNet Biotech offers a full suite of protein interaction validation services, including yeast two-hybrid, Co-IP, and GST pull-down assays.

Services Workflow

Online Consultation

Solution Matching

Service Contract

One-Stop-Services

Project Report

Any question? Get in touch with us!