Services
Providing Advanced Biological Technical Services for Researchers in Fundamental Science
SPR Molecular Affinity Analysis Service
High-Sensitivity SPR Affinity Analysis Service for Biomolecular Interactions
Overview
ProNet Biotech now offers high-precision Surface Plasmon Resonance (SPR) molecular affinity analysis using the Biacore T200 system, supported by our proprietary AI-powered digital interaction prediction platform. This label-free, real-time biosensing method delivers high-sensitivity and rapid quantitative results across a wide range of biomolecular interactions.
Application Areas
· Quantitative analysis of interactions between small molecules, proteins, peptides, and nucleic acids
· Inhibitor screening
· Antibody screening and characterization
Key Advantages
Gold Standard for Interaction Analysis: SPR is widely recognized as the most reliable technique for detecting biomolecular interactions.
Biacore T200 Platform: Label-free detection, ultra-sensitive, capable of detecting low-affinity interactions with rapid response time.
Data Accuracy: All assays are conducted under strict quality control to ensure precise kinetic and affinity results.
Versatile Detection: Suitable for interactions involving proteins, small molecules, nucleic acids, and peptides.
AI Integration: Combined with our AI-powered digital prediction platform for a one-stop solution from interaction prediction to validation.
Service Workflow
Interaction prediction (optional) → Conjugated ligand → Sample submission → SPR assay setup and optimization → Real-time binding analysis → Data processing and kinetic modeling → Final report delivery
Service Details
Service Item | Turnaround | Deliverables |
|---|---|---|
Multi-concentration SPR Affinity Analysis | 7 business days | Experiment Report |
Single-concentration SPR Affinity Detection |
Sample Requirements
Sample Type | Requirements | Shipping Conditions |
|---|---|---|
Protein | ≥90% purity, ≥200 μg total, ≥1 mg/mL. Buffer should not contain glycerol, urea, Tris, or other high refractive index substances. Preferred buffer: 0.01M HEPES, 0.15M NaCl, 0.003M EDTA, 0.05% Tween 20, pH 7.4 | Dry ice |
Small Molecule | Powder or solution, ≥1 mg, ≥90% purity, ≥500 μM water solubility. Dissolved in PBS, HEPES, or water | Ice pack |
Nucleic Acid | ≥5 OD, shipped as dry powder | Ice pack |
Case Example: Antigen–Antibody Interaction
The positive control (Anti-His mAb, clone 1C5) demonstrated significant binding with PD-RBD-His, while other tested samples showed no clear interaction signals.
Figure 1: Sensorgram of analyte–ligand interaction kinetics.
Statement: Services sold by our company are intended for research purposes only and must not be used for diagnostic or therapeutic purposes.
Services Workflow
Online Consultation
01
Solution Matching
02
Service Contract
03
One-Stop-Services
04
Project Report
05
Related Products
Product Inquiry
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
Need more info?
Let's connect!