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Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

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Interaction Validation Platform

Tobacco Genetic Transformation

Project Brief

The explants (callus) of tobacco were co-cultured with Agrobacterium. During the co-culture process, Agrobacterium adhered to the surface of tobacco explant cells and transferred T-DNA into the tobacco cells. The target gene carried by T-DNA would randomly integrate into the genome of tobacco cells. Finally, by utilizing the totipotency of plant cells and through tissue culture techniques, the transformed tobacco cells were cultivated into complete transgenic plants.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	Service Period	Delivery Standard (Default delivery includes resistance gene identification)
cigarettes and tobacco	1. Vector construction / Gene synthesis 2. Sequencing of the target gene + Vector construction 3. The target gene has been constructed into an expression vector and its sequence has been verified through sequencing.	nicotiana benthamiana	ten	1. Vector Construction: The client does not provide the vector template and does not deliver it. 2. Gene Synthesis: The client does not provide the vector template and does not deliver it.
	overexpression		3 to 4 months	The number of T0 generation positive plants (resistant plants) is ≥ 10.
			6 to 7 months	T1 seed（0.2-0.3g）
			9 to 10 months	More than 5 T1-positive strains
			10.5 - 11.5 months	T2 seeds（0.2-0.3g）
			13.5 - 14.5 months	More than 5 T2-positive strains
			15 - 16 months	T3 Seed（0.2-0.3g）
			18-19 months	More than 5 T3-positive strains
	Single-gene knockout		4 to 5 months	The number of T0 generation positive seedlings (resistant seedlings) is ≥ 3.
			7 to 8 months	T1 Seed（0.2-0.3g）
			10 to 11 months	More than 3 T1-positive strains
			11.5 - 12.5 months	T2 seeds（0.2-0.3g）
			14.5 - 15.5 months	More than 3 T2-positive strains
			16 - 17 months	T3 seeds (0.2 - 0.3 grams)
			19th - 20th month	More than 3 T3-positive strains

Note: Other varieties can be tested*.

Case Show

From left to right and from top to bottom, they are pre-culture, screening, differentiation, rooting, and emergence of seedlings.

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