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Pumpkin Genetic Transformation

Project Brief

Pumpkins belong to dicotyledonous plants. The genetic transformation technology of pumpkins is achieved through artificial-mediated methods, where exogenous target genes (such as insect-resistant, disease-resistant, and stress-tolerant genes) are introduced into pumpkin cells, enabling their stable integration, replication and expression within the cells. Eventually, transgenic pumpkin plants with new genetic traits are obtained.

Technological Superiority

1. Efficient screening: Strict optimization of each step in genetic transformation, resulting in high transformation efficiency.

2. Full-process monitoring: From seed disinfection onwards, every stage is strictly monitored with real-time progress tracking.

3. Integrated quantitative analysis: Offers detection methods such as PCR, qPCR, and Sanger sequencing to ensure authentic transgenic outcomes.

Sample Receiving Standards

Type	Sample Submission Standards	Conditions of Carriage
The sequence that requires gene synthesis	nothing	nothing
The constructed plasmid Intermediate vector plasmid	Concentration > 100 ng/ul, Volume > 20 ul; Provides carrier sequence and resistance, without contamination.	The plasmid will be sent using an ice pack.
Contains bacterial culture with the constructed plasmid; contains bacterial culture with the intermediate vector plasmid	Provide resistance. For bacterial solutions with a volume greater than 0.5ml and glycerol bacteria that have been stored for more than one year, the samples should be activated before submission.	The bacterial liquid is sent by dry ice. The amount of dry ice used: 1 day (5kg), 2-3 days (10-20kg), 3-5 days (20-30kg).
Planktonic bacteria	non-pollution	normal temperature
Note: 1. For other types of samples, please contact our company in advance. 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Service Process, Cycle and Delivery

Species	Type of Service	Variety	Service Period	Delivery Standard (Default delivery includes resistance gene identification)
pumpkin	1. Vector construction / Gene synthesis 2. Sequencing of the target gene + Vector construction 3. The target gene has been constructed into an expression vector and its sequence has been verified through sequencing.	Chinese pumpkin, Jingxin Jian 2 variet	ten	A. Vector Construction: The client does not provide the vector template and does not deliver it. B. Gene Synthesis: The client does not provide the vector template and does not deliver it.
	overexpression		5 to 6 months	The number of T0 generation positive plants (resistant plants) is ≥ 10.
	overexpression		10 to 12 months	Two T0 positive seedlings were harvested to obtain T1 mature fruits (a total of 2 samples, the exact weights are subject to actual measurement)
	Single-gene knockout		6 to 7 months	The number of T0 generation positive plants (resistant plants) is ≥ 5.
	Single-gene knockout		11 to 13 months	One T0 positive seedling was harvested to produce T1 mature fruits (a total of 1 sample, the exact weight is subject to actual measurement)

Note: Other varieties can be tested*.

Case Show

A. Seed induction B. Callus isolation C. Co-culture D. Regeneration E. Screening F. Differentiation G. Rooting H. Identification

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