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Providing Advanced Biological Technical Services for Researchers in Fundamental Science
Subcellular Localization Service
Subcellular Localization Services for Protein and Gene Expression Analysis
▼ Subcellular Localization Service Overview
Subcellular localization refers to the specific positioning of a protein or gene expression product within a cell, such as in the nucleus, cytoplasm, cell membrane, or specific organelles. Understanding the distribution of proteins within these compartments is vital for the initial assessment of their functional roles. Our subcellular localization services help identify where proteins are located within the cell, providing valuable insights into their biological functions.
▼ Transformation Systems for Subcellular Localization Studies
We offer various genetic transformation systems for precise subcellular localization analysis, including:
· Monocot Rice Protoplast Transformation System: Suitable for protein localization studies in rice cells.
· Dicot Arabidopsis Protoplast Transformation System: For research on model plant species like Arabidopsis.
· Tobacco Transformation System: Provides detailed protein localization analysis in tobacco cells.
Co-localization Marker Vectors:
We provide marker vectors designed for protein localization to various cellular compartments, including:
· Nucleus
· Cell membrane
· Vacuole membrane
· Transport vesicles
· Endoplasmic reticulum
· Golgi apparatus
· Mitochondria
· Chloroplasts
Each marker is equipped with multiple fluorescent proteins, allowing for co-localization studies and visualization in combination with other markers.
▼ Subcellular Localization Service Workflow
Our streamlined workflow ensures efficient and accurate subcellular localization analysis:
Gene Cloning and Vector Construction: We construct expression vectors tailored to your protein or gene of interest.
Transformation and Expression: Utilizing our robust transformation systems, we express your target protein in selected cells.
Subcellular Localization Imaging: We use advanced confocal microscopy to visualize protein localization with high-resolution images.
Analysis and Reporting: A comprehensive report will be provided, including experimental procedures, results, and confocal images suitable for publication.
▼ Subcellular Localization Service Contents & Timeline
Gene Synthesis/Vector Construction (10 business days): Raw data, result images, and standard experiment reports.
Agrobacterium Transformation & PCR Verification (7 business days): Agrobacterium transformation and PCR positive verification.
Plant Growth and Transfection (7 business days): Transfection of rice protoplasts, Arabidopsis protoplasts, and tobacco cells.
Confocal Microscopy (1 business day): High-resolution confocal images for subcellular localization analysis.
▼ Subcellular Localization Project Delivery Standards
We adhere to the highest standards for project delivery, ensuring every client receives complete, high-quality, and publication-ready results. Our deliverables include:
· One aliquot of the recombinant plasmid containing your target gene (Optional);
· Sequencing results of the recombinant plasmid to verify successful construction;
· Original experimental images, including:
· For co-localization experiments: 5 images per sample (fluorescence image of the target protein, marker fluorescence image, merged fluorescence image of target and marker, bright-field image, and full overlay);
· For standard localization experiments: 3 images per sample (target protein fluorescence image, bright-field image, and merged image);
· A standard experimental report, which includes:
· Detailed experimental procedures;
· Full workflow overview;
· Final results in a structured and publication-ready format;
These materials are provided to support your research, facilitate data analysis, and meet SCI publication standards for subcellular localization studies.
▼ Subcellular Localization Case Studies
Here is an example of subcellular localization in rice protoplasts, demonstrating the use of our markers and imaging techniques for precise protein localization:
Example Images: Confocal images showcasing protein localization in tobacco:
▼ Key Benefits of Our Subcellular Localization Services
Precise Localization: Pinpoint where your protein is located within the cell to understand its functional role.
Multiple Compartment Targets: Our service covers a wide range of cellular compartments for detailed analysis.
High-Resolution Imaging: Using cutting-edge confocal microscopy, we ensure the highest quality of imaging.
Comprehensive Reports: SCI-quality images and detailed experimental reports ensure your research is publication-ready.
▼ Get Started with Our Subcellular Localization Services Today!
Interested in discovering where your proteins are located within the cell? Contact us below to discuss your project and get a customized quote for our subcellular localization services. Our team is here to support your research and provide high-quality, reliable results.
Services Workflow
Online Consultation
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Solution Matching
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Service Contract
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One-Stop-Services
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Project Report
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Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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