Enhance your research with the Yeast Abiotic-Stress Resistance Gene Screening Vector Kit. Includes four yeast strains and the pYES2-NTB vector for screening under various stress conditions like salt, drought, and temperature.

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Product Description

Yeast Abiotic-Stress Resistance Gene Screening Vector Kit – Screening Kit for Salt, Drought, Temperature & More

Product Overview

The Yeast Abiotic-Stress Resistance Gene Screening Vector Kit includes four yeast strains—INVSc1, BY4741, Δycf1, and W303—each compatible with the pYES2-NTB vector. This versatile kit is designed for gene screening under various abiotic stress conditions, including salt, drought, high/low temperature, and more. The pYES2-NTB vector features the inducible GAL promoter, which drives high levels of exogenous protein expression in the presence of galactose, while suppressing expression in glucose conditions. The vector also contains the URA3 gene for selection using SD-URA plates.

This kit is ideal for conducting single-gene validation assays and large-scale screening of stress-related genes. Whether you are researching abiotic stress tolerance or developing stress-resistant traits, the Yeast Abiotic-Stress Resistance Gene Screening Kit provides all the necessary components for successful experimentation.

CAT: RY8013

Price and Instruction: Available upon inquiry

Product Specifications

Component	Name	Concentration	Specification	Quantity
Plasmid	pYES2-NTB	100 ng/μL	20 μL	1 tube
Strain	INVSc1	Inoculated Plate	Diameter:90mm	1 plate
Strain	BY4741	Inoculated Plate	Diameter:90mm	1 plate
Strain	Δycf1	Inoculated Plate	Diameter:90mm	1 plate
Strain	W303	Inoculated Plate	Diameter:90mm	1 plate
Primer	pYES2-NTB-F	Lyophilized powder	2 OD	1 tube
Primer	pYES2-NTB-R	Lyophilized powder	2 OD	1 tube

Storage and Shipping Conditions

Plasmids: Store at -20°C, shipped at room temperature.

Yeast Strains: Store at room temperature, shipped at room temperature.

Primers: Store at -20°C, shipped at room temperature.

Experimental Guidelines

Yeast Strain Selection

INVSc1: Suitable for salt and drought stress screening.

BY4741: Suitable for high and low temperature stress screening.

Δycf1: Suitable for cadmium stress screening.

W303: Suitable for pesticide-related stress screening.

Experimental Procedure

Product Advantages

High-Quality Yeast Strains: Includes four distinct yeast strains optimized for screening under a range of abiotic stress conditions.

Inducible Expression Vector: The pYES2-NTB vector allows for controlled protein expression, making it ideal for gene screening and functional studies.

Versatile Application: Suitable for single-gene validation and stress-related gene library screening.

Precautions

1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.

2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.

3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.

4. Maintain sterile conditions to avoid contamination.

Self-prepared Materials

1. Sterile PCR tubes, tips, centrifuge tubes, shakers, incubators, and reagents like 50% PEG3350, 1M LiAc, X-α-Gal, and 3-AT.

2. (Optional from Pronetbio) Yeast media (YPDA, SD-Trp, SD-Trp/-Leu/-His), yeast plasmid extraction kits, and positive clone amplification kits.

FAQs

Q1: What is the Yeast Abiotic-Stress Resistance Gene Screening Kit used for?
The Yeast Abiotic-Stress Resistance Gene Screening Kit is used for screening genes related to abiotic stress resistance, including salt, drought, temperature extremes, and more. It is ideal for researchers studying stress tolerance genes in yeast models.

Q2: Can I use the Yeast Abiotic-Stress Resistance Gene Screening Kit for multiple screenings?
Yes, the kit is reusable for various screenings, including single-gene validation and large-scale stress library screening.

Q3: What yeast strains are included in the kit, and what stress conditions are they suitable for?
The kit includes four yeast strains: INVSc1 (salt and drought stress screening), BY4741 (high/low temperature stress screening), Δycf1 (cadmium stress screening), and W303 (pesticide-related stress screening).

Q4: How do I activate the yeast strains after receiving them?
For glycerol-stored strains, thaw them at 4°C, streak onto YPDA plates, and incubate at 28°C for 2-3 days.
For plate-stored strains, directly streak onto YPDA plates and incubate at 28°C for 2-3 days.

Q5: Can I use the pYES2-NTB vector for other types of gene expression studies?
Yes, the pYES2-NTB vector is ideal for protein expression in yeast, especially for studies involving abiotic stress-related genes.

Q6: How should I store the components of the kit?
Plasmids and primers should be stored at -20°C.
Yeast strains should be stored at room temperature but protected from contamination.

Contact Us

For further inquiries or technical support, feel free to contact our customer service team below. We are here to assist you with your research needs.

Why Choose Us?

At Pronetbio, we specialize in providing high-quality, reliable research tools for molecular biology. Our products are designed to streamline your research and ensure accurate, reproducible results. With the Yeast Abiotic-Stress Resistance Gene Screening Vector Kit, you can accelerate your stress-related gene research and discover the mechanisms behind abiotic stress tolerance.

Statement: All products are for research purposes only. Not for use in diagnostic or therapeutic applications.

Keywords: Abiotic Stress Screening, Yeast Stress Screening Kit, Protein Expression Assay, GAL Promoter, pYES2-NTB Vector, Salt Stress Screening, Drought Stress Screening, High Temperature Stress, Cadmium Stress, Pesticide Stress, Single-Gene Stress Validation.

Tel: +86 025-85205672

Email: info@pronetbio.com

Address: Building 3C, Nanjing Xianlin Zhigu,

Qixia District, Nanjing, Jiangsu, China, 210033

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