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Yeast One Hybrid (TF-Centered) Vector Kit
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Discover the TF-Centered Yeast One-Hybrid (Y1H) Vector Kit, a powerful tool for identifying transcription factor (TF) recognition elements with high efficiency and accuracy. Featuring a comprehensive 7-bp randomized DNA library, this kit supports high-throughput protein-DNA interaction analysis, making it ideal for transcriptional regulation research. Perfect for both basic and applied studies.
INSTRUCTION & COA：
- Product Description
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Product Overview
The TF-Centered Yeast One-Hybrid (Y1H) Vector Kit is designed to identify transcription factor (TF) recognition elements efficiently and accurately. Based on the principle of GAL4 AD-mediated activation of the reporter gene HIS3, this system enables researchers to uncover interactions between proteins and DNA by facilitating growth on 3-AT-containing media.
This kit includes a randomized 7-bp DNA motif library containing 3×4⁷ sequences, enabling robust identification of transcription factor binding sites. It supports high-throughput and reliable experiments, offering flexibility across diverse research scenarios in transcription factor-DNA interaction studies.
CAT: RY8012
Price and Instruction: Available upon inquiryKey Features
Comprehensive Coverage: The randomized DNA motif library contains all possible 7-bp sequences, ensuring compatibility with various transcription factors and species.
High Efficiency: Accurately identifies TF recognition elements in a fast and straightforward workflow.
Reliable System: Includes positive controls (pHIS2-p53 + pGAD53m) and precise plasmid and primer combinations for reproducibility.
Flexible Application: Ideal for both basic and applied research in transcriptional regulation.
Product Components
Component
Name
Specification
Volume
Quantity
Plasmid
pGADT7
100 ng/μL
20 μL
1 tube
Plasmid
pHIS2
100 ng/μL
20 μL
1 tube
Plasmid
pHIS2-p53
100 ng/μL
20 μL
1 tube
Plasmid
pGAD53m
100 ng/μL
20 μL
1 tube
Strain
Promoter Library Yeast
Suspension
1 mL
4 tube
Strain
Y187
Inoculated Plate
1 plate
1 plate
Primer
pHIS2-F
Lyophilized powder
2 OD
1 tube
Primer
pHIS2-R
Lyophilized powder
2 OD
1 tube
Primer
AD-R
Lyophilized powder
2 OD
1 tube
Primer
T7-F
Lyophilized powder
2 OD
1 tube
Experimental Guidelines
Storage and Shipping
Item
Storage Condition
Shipping Condition
Plasmids
-20°C
Room Temperature
Strains
Room Temperature
Room Temperature
Primers
-20°C
Room Temperature
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
FAQs
Why is self-activation testing necessary?
Self-activation ensures the reporter gene is not expressed due to endogenous transcription factors or promoter leakage, providing reliable results.
Can this kit be reused?
Yes, the plasmids and yeast strains in the kit can be expanded and preserved for repeated use.
Why is this system preferred over other Y1H systems?
Unlike EGY48-LacZ systems, this kit does not require additional staining reagents, making it simpler and faster to use.
Self-prepared Materials
1. Sterile PCR tubes, tips, centrifuge tubes, shakers, incubators, and reagents like 50% PEG3350, 1M LiAc, X-α-Gal, and 3-AT.
2. (Optional from Pronetbio) Yeast media (YPDA, SD-Trp, SD-Trp/-Leu/-His), yeast plasmid extraction kits, and positive clone amplification kits.
Why Choose Us?
Proven Results: Trusted by researchers worldwide for high-throughput and reliable transcription factor studies.
Integrated Solutions: Compatible with our Yeast Colony Rapid Detection Kit and Yeast Plasmid Extraction Kit for enhanced workflow efficiency.
Expert Support: Access our scientific team for consultation and troubleshooting.
Ready to Advance Your Research?
Ready to enhance your transcription factor research?Contact us today for a detailed consultation and product quotation. Let us help you unlock new insights in molecular interaction research. When inquiring about this product, please reference Product Code: RY8012.
Statement: All products are for research purposes only. Not for use in diagnostic or therapeutic applications.
Related Service: TF-Centered Yeast One-Hybrid (Y1H-TF) Screening Service (Service Code: SRY8012)
Keywords: Yeast One-Hybrid TF vector kit, Transcription factor binding motif identification, 7-bp randomized DNA library, Protein-DNA interaction analysis, Efficient Y1H experimental system.
Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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