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2025-05-20
This study reveals that both phytochrome A (phyA) and phytochrome B (phyB) interact with PHYTOCHROME-INTERACTING FACTORs (PIFs) through the APA (active phyA-binding) motif via the C-terminal output module (phyOPM), rather than the previously assumed N-terminal photosensory module (phyPSM). While phyB also binds the APB (active phyB-binding) motif via its N-terminal phyBPSM, the APA motif interaction is common to both phyA and phyB through phyOPM.
2025-05-16
This study revealed that the transcription factor ZmGBF1 plays a positive role in maize heat stress (HS) tolerance. Through transcriptome analysis, DNA affinity purification sequencing, yeast one-hybrid, and dual-luciferase assays, ZmGBF1 was shown to directly bind to the promoter of ZmCXE2 and enhance its expression, thereby promoting the HS response. Both ZmGBF1 and ZmCXE2 were upregulated by gibberellin (GA), suggesting hormonal involvement in this regulatory pathway. The findings propose an improved model in which ZmGBF1 enhances maize heat tolerance by activating ZmCXE2, helping to balance growth and environmental stress responses.
2025-05-13
This study investigates the regulatory mechanisms of fig fruit textural changes during ripening. It focuses on the transcription factor FcERF100, which plays a key role in repressing fruit softening. FcERF100 transcription is rapidly suppressed during fig fruit ripening, and its overexpression delays softening by decreasing the expression of the cell wall-modifying gene FcPL7. Through various assays (Y1H, ChIP-qPCR, EMSA, and dual-luciferase), it was shown that FcERF100 represses FcPL7 by binding directly to its promoter via specific elements (GCC-box and DRE/CRT).
2025-05-09
Dehydrins (DHNs), part of the LEA D11 family, are hydrophilic proteins known for their role in plant responses to abiotic stresses, but their functions in cucurbit crops remain largely unexplored. In this study, 34 DHN genes were identified across six cucurbit species, including Cucumis melo. The genes showed high collinearity within subfamilies. Different DHN genes exhibited varying responses to abiotic stresses such as cold, salt, cadmium, and notably aluminum.
2025-05-07
This study explores the molecular mechanism underlying bud dormancy release in tree peony, with a particular focus on the gibberellin (GA) signaling pathway. Researchers identified PsAGL9, a MADS-box transcription factor, as an interacting partner of PsRGL1, a DELLA protein that negatively regulates dormancy release. PsAGL9 expression was induced by both chilling treatment and exogenous GA₃ application, indicating its potential role in the GA-mediated dormancy process.
2025-04-29
Drought severely impacts plant growth and productivity, yet the regulatory networks behind drought responses at the cellular level remain unclear. This study presents a comprehensive multi-omics integration framework-combining transcriptomic, proteomic, epigenetic, and network analyses-to map cell-type-specific regulatory networks during drought.
2025-04-25
This study uncovers the transcriptional regulatory network controlling SmCYP71D375, a key enzyme involved in tanshinone biosynthesis in Salvia miltiorrhiza. Using the promoter of SmCYP71D375 as bait in a yeast one-hybrid screen, researchers identified SmMYB53, an R2R3-MYB transcription factor, as an upstream regulator. Overexpression of SmMYB53 in transgenic hairy roots increased SmCYP71D375 expression and promoted tanshinone accumulation, while RNAi-mediated silencing of SmMYB53 reduced it.
2025-04-22
A Comprehensive Overview – Dual-Luciferase Reporter Gene Assay
The dual-luciferase reporter gene assay employs Firefly luciferase as the reporter gene and Renilla luciferase as the internal control gene. By simultaneously detecting the activity of both luciferases, this system enables accurate quantitative analysis of target gene expression or molecular interactions.
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