Single chain antibody fragment (scFv) is a small molecule antibody in the third generation of antibodies, which is formed by connecting the heavy chain variable region and light chain variable region of the antibody through a short peptide (linker) of 15-20 amino acids.
Nanjing Ruiyuan Biotechnology is committed to using faster growing yeast to replace mammalian cells represented by CHO for antibody production and development of cheaper antibody drugs, with characteristics such as low relative molecular weight, good tissue permeability, strong specificity, low immunogenicity, and short half-life.
Pichia pastoris is a methanol trophic yeast that encodes two alcohol oxidase (AOX) genes, AOX1 and AOX2, with similar sequences. The AOX1 gene is strictly induced and regulated by methanol. When methanol is the sole carbon source, the AOX1 promoter can be induced by methanol to activate the expression of ethanol oxidase, thereby metabolizing with methanol. Plasmids containing the AOX1 promoter can be used to promote the expression of target genes encoding foreign proteins.
Advantages of Ruiyuan Biological Services
1. Using yeast as an expression vector for antibodies allows for faster construction of cell lines during the antibody development process, eliminating time-consuming virus clearance validation steps, and greatly saving the development cycle.
At the same time, during the antibody production process, the fermentation cycle can be significantly shortened and high-density large-scale cultivation (up to 160000 liters) can be achieved.
2. A high-throughput screening platform can quickly and effectively obtain the optimal expression conditions;
3. Ruiyuan Biology has a professional research team and mature production line in the yeast expression system.
Ruiyuan Biological researchers have established a mature yeast expression and purification service platform, providing high-quality expression and purification services for small molecule antibodies in Pichia pastoris