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Nanoantibody screening

Yeast Two-Hybrid Y2H
Mb Y2H for Membrane Proteins

Yeast one-Hybrid Y1H
TF-centered YlH
Yeast Three-Hybrid Y3H

Abiotic-Stress Resistance Gene Screening

Digital Analog Library Screening

Products
Peptide Library Screening

Peptide Library Screening

Peptides are synthetically accessible, amenable to chemical tailoring, and have the potential to bind the typically shallow surfaces seen in therapeutically relevant—and historically intractable—protein–protein interactions (PPIs). Peptides can either act as natural ligands in the form of cofactors, coenzymes, and hormones, or directly interact with macromolecules including proteins, RNA, or DNA.

Nanobody Screening Vector Kit

Nanobody Screening Vector Kit

The antigen-binding capacity of nanobodies is similar to conventional antibodies for three reasons. First, the complementarity-determining region 3 (CDR3) of nanobodies is similar or even longer than that of human VH domain (variable domain of heavy immunoglobulin chain). Second, nanobodies can form finger-like structures to recognize cavities or hidden epitopes that are not available to mAbs.

Saccharomyces cerevisiae Surface Display Vector Kit

Saccharomyces cerevisiae Surface Display Vector Kit

The most common yeast display system employs fusion of the protein of interest to the C-terminus of the a-agglutinin mating protein Aga2p subunit, a technology pioneered by Boder and Wittrup. The yeast surface display construct designed for this system includes two epitope tags: a hemagglutinin (HA) tag between Aga2p and the N-terminus of the protein of interest, and a C-terminal c-myc tag.

Yeast Abiotic-Stress Resistance Gene Screening Vector Kit

Yeast Abiotic-Stress Resistance Gene Screening Vector Kit

Saccharomyces cerevisiae belongs to eukaryote, which is closer to the expression system of plants and animals. This expression system includes the following processes: glycosylation, disulfide bond formation, and Post-translational modification of protein folding.

Yeast Colony Rapid Detection Kit

Yeast Colony Rapid Detection Kit

This kit is used to amplify fragments from the yeast genome as well as transformed plasmids. The component contains a visualization green dye, which can directly perform polyacrylamide gel electrophoresis and agarose gel electrophoresis after PCR. The PCR product has A- tailing at the 3' end, which is suitable for TA cloning.

Yeast One Hybrid (Gene-Centered) Vector Kit

Yeast One Hybrid (Gene-Centered) Vector Kit

Yeast one-hybrid system is a technique developed from yeast two-hybrid to study DNA-protein interactions, and is widely used to study the regulation of gene expression in eukaryotic cells, such as identifying DNA binding sites to discover potential binding protein genes, analyzing DNA binding structural domain information, etc.

Yeast One Hybrid (TF-Centered) Vector Kit

Yeast One Hybrid (TF-Centered) Vector Kit

TF-centered YIH (Yeast One-Hybrid Technology), which recognizes transcription factors as its recognition elements, can accurately and efficiently identify transcription factor recognition elements, and has a wide range of applications in the study of protein-DNA element interactions.

Yeast Secreted Protein Expression Vector Kit

Yeast Secreted Protein Expression Vector Kit

One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are used for the production of recombinant proteins including bacteria, yeasts, molds, mammals, plants, and insects.

Yeast Signal Peptide Detection Vector Kit

Yeast Signal Peptide Detection Vector Kit

Currently, the most widely used method for prediction of signal peptide from amino acid sequences is to use software SignalP (Petersen et al., 2011). Yeast signal sequence trap (YSST) is a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast.

Yeast Three-Hybrid (Nuclear System) Vector Kit

Yeast Three-Hybrid (Nuclear System) Vector Kit

The yeast three-hybrid system is mainly used to study more complex macromolecular interactions including three components, providing a new method for the study of protein-protein-protein, protein-RNA-protein, and protein-small molecule-protein interactions.

Yeast Two-Hybrid (Membrane System) Vector Kit

Yeast Two-Hybrid (Membrane System) Vector Kit

DUAL membrane system designed to identify interactions involving integral membrane proteins or membrane-associated proteins is derived from Dualsystems Biotech AG.

Yeast Two-Hybrid (Nuclear System) Vector Kit

Yeast Two-Hybrid (Nuclear System) Vector Kit

The yeast two-hybrid system is a research method for identifying and detecting protein interactions in living cells, which is performed in the eukaryotic model organism yeast, and is now used in several research fields because of its high sensitivity and wide applicability.

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