Services

Providing Advanced Biological Technical Services for Researchers in Fundamental Science

Interaction Screening Platform

Molecular Application Platform

AI Digital Platform

Interaction Validation Platform

Pull down MS

Service Description

Pull-down MS is a combination of two techniques used to study protein-protein interactions;

Pull down: A technique that uses a “bait” molecule to specifically “fish out” interacting “prey” molecules—either directly or indirectly—from complex biological samples, such as cell lysates.

MS (Mass Spectrometry): Utilizing mass spectrometry techniques to qualitatively and quantitatively analyze the captured “prey", thereby identifying novel interacting proteins.

Application Areas

Protein interaction screening and drug target discovery.

Technical Advantages

High sensitivity: capable of detecting low-abundance interacting proteins;

High coverage: simultaneously identify hundreds of potential interacting proteins in a single experiment;

Highly flexible: suitable for experimental designs involving various tagging systems (GST, His, MBP, etc.);

Professional analysis: Provides in-depth bioinformatics interpretation (including differential protein screening, expression pattern clustering analysis, interaction network analysis, etc.).

Service Process, Cycle, and Delivery

Service Content	Weekday (working day)	Delivery Content
Optional Services: 1. Gene synthesis (with optimized codons / without optimized codons) 2. Sequencing of the target gene + Construction of the vector 3. The target gene has been constructed into the pET28a-His/pET32a-T1xA-His/pGEX-6P-1-GST/PET28a-MBP vector, and the target gene has been sequenced for verification. 4. Prokaryotic protein expression	29	1.Plasmid/remaining protein; 2. Experimental related original images: images of SDS-PAGE identification for prokaryotic expression and pull-down WB identification; 3. Standard experimental process report.
Pull down 1. Extract total protein 2. Incubate the successfully expressed protein with the total protein 3. Protein Purification 4. WB Identification	12
LC-MS mass spectrometry analysis	24
Aggregate	65

Sample Acceptance Criteria

Type	Standards	Conditions Of Carriage
The sequence requiring gene synthesis	——	——
The constructed plasmid The intermediate vector plasmid	Concentration > 100 ng/μL, volume > 20 μL; provide vector sequence and resistance, without contamination.	Ice bag
The E. coli culture containing the constructed plasmid The E. coli culture containing the intermediate vector plasmid	Volume > 500 μL; provides vector sequence and resistance, and is free of contamination	Dry ice delivery: Dry ice usage: 1 day (5 kg), 2-3 days (10-20 kg), 3-5 days (20-30 kg).
The E. coli plate culture	Non-pollution	Room temperature.
Antibody Purified target protein	IP level antibody > 30 μL	Dry ice delivery: Dry ice usage: 1 day (5 kg), 2-3 days (10-20 kg), 3-5 days (20-30 kg).
Material (for extracting total protein)	>5 g
Total protein sample	>0.5 mL
The purified target protein	>30 μL
Note: 1. For other types of samples, please contact our company in advance; 2. For dry ice sampling and transportation, notify our company at least 2 days before submitting the samples.

Case Show

Protein expression in prokaryotes

Select the correct cloned samples that have been saved and examine the expression of the target protein. The results show that the target protein was successfully expressed.