ABOUT US

Your Trusted Partner

Company profile

Company Culture

R&D Strength

News

Optimization : Extracellular Affinity Maturation by Yeast Display

Release time:

2023-04-16

In vitro affinity maturation of nanobodies and scFv.

We use a combination of mutagenesis and yeast display to greatly improve the binding affinity of your nanobody or scFv. A mutant VHH or scFv library (2-5 x 106) derived from the parental sequence is generated by introducing mutations either across the entire length of the antibody sequence or restricted to the CDR regions and cloned into our proprietary vector for yeast surface expression.
Yeast expressing the mutant library is subjected to several rounds of cell sorting and the concentration of antigen is gradually decreased to select nanobodies with the highest binding affinities. Our yeast display platform controls for the antigen-antibody equilibrium, which is a major advantage compared to phage display.

Key benefits
● Cost-effective and quick optimization method
● No drift-effect like in phage display
● Apparent kD measurements

Related News

2023-12-10

Targeting Protein-Protein Binding for Therapeutic Development: Unlocking the Potential of Biologics

Table of Contents 1. Introduction 2. Understanding Protein-Protein Binding 2.1 Anatomy of Protein-Protein Interactions 2.2 Significance in Drug Discovery 2.2.1 Targeting Protein-Protein Binding: A Paradigm Shift 2.2.2 Advantages of Protein-Protein Binding as a Therapeutic Strategy 3. Techniques for Studying Protein-Protein Binding 3.1 X-ray Crystallography 3.2 Nuclear Magnetic Resonance (NMR) Spec

2023-12-10

Understanding Protein Protein Binding and Its Significance in Medicine

Table of Contents: 1. Introduction: Unraveling the Mysteries of Protein Protein Binding 2. The Fundamentals of Protein Protein Binding 3. Protein Protein Binding in Drug Discovery and Development 4. Targeting Protein Protein Interactions for Therapeutic Purposes 5. Protein Protein Binding and Disease Pathways 6. Challenges and Advances in Studying Protein Protein Binding 7. FAQs: Clarifying Common

2023-12-10

Understanding Protein Protein Binding: A Comprehensive Guide

Table of Contents: 1. Introduction: The Significance of Protein-Protein Binding 2. The Basics of Protein Structure and Function 3. Understanding Protein-Protein Interactions 4. Mechanisms of Protein-Protein Binding 4.1 Covalent Binding 4.2 Non-covalent Binding 4.3 Electrostatic Interactions 4.4 Hydrophobic Interactions 4.5 Van der Waals Forces 4.6 Conformational Changes 5. Experi

2023-12-10

The Role of Protein Protein Binding in Drug Discovery: A Comprehensive Guide

Table of Contents: 1. Introduction: Understanding Protein-Protein Binding 2. The Significance of Protein-Protein Binding in Drug Discovery 3. Mechanisms of Protein-Protein Binding 3.1. Hydrophobic Interactions 3.2. Electrostatic Interactions 3.3. Van der Waals Forces 4. Challenges in Targeting Protein-Protein Interactions 4.1. Protein-Protein Interfaces 4.2. Binding Site Identificat

2023-12-10

Exploring the Mechanisms of Protein-Protein Binding: Unlocking the Secrets of Biochemical Interactions

Table of Contents 1. Introduction: Unveiling the Significance of Protein-Protein Binding 2. Understanding the Structure of Proteins 3. Recognizing the Importance of Protein-Protein Interactions 4. Key Factors Influencing Protein-Protein Binding 4.1. Electrostatic Interactions: The Power of Opposites 4.2. Hydrophobic Interactions: Like Dissolves Like 4.3. Van der Waals Forces: Attractive I

2023-12-09

Unveiling the Secrets of Protein-Protein Interaction: A Breakthrough Study

Table of Contents: 1. Introduction 2. Understanding Protein-Protein Interaction 3. The Significance of Protein-Protein Interaction in Biological Systems 4. Methods Used in Studying Protein-Protein Interaction 5. Key Findings from the Breakthrough Study 6. Implications and Applications in Medicine and Biotechnology 7. Frequently Asked Questions (FAQs) 8. Conclusion 1. Introduction Proteins are the

2023-12-09

Revolutionizing Drug Discovery through Protein Protein Interaction: An Innovative Approach to Advancing Pharmaceutical Research

Table of Contents 1. Introduction: Unleashing the Power of Protein Protein Interaction 2. Understanding the Basics of Protein Protein Interaction 3. The Significance of Protein Protein Interaction in Drug Discovery 4. Exploring the Techniques for Studying Protein Protein Interaction 5. Applications of Protein Protein Interaction in Drug Discovery 6. Overcoming Challenges in Protein Protein Interac

2023-12-09

The Significance of Protein-Protein Interaction in Biopharmaceutical Research: Uncovering the Secrets of Drug Development

Table of Contents: 1. Introduction: Understanding Protein-Protein Interaction 2. The Importance of Protein-Protein Interaction in Biopharmaceutical Research 3. Protein-Protein Interaction as a Key Driver in Drug Discovery 3.1 Protein Complexes: Paving the Way for Targeted Therapies 3.2 Unveiling Disease Mechanisms Through Interactome Analysis 3.3 Protein-Protein Interaction Networks: Mapp

Do you have a question for us?

contact our experts

Explore More →

Tel: +86 025-85205672

Email: info@pronetbio.com

Room 1001, Floor 10, Building 3C, Nanjing Xianlin Zhigu, No. 6 Qimin West Road, Xianlin Street, Qixia District, NanJing, Jiangsu, China, 210033

Subscribe newsletter

Business license

Any question?

Get in touch with us!

Any question? Get in touch with us!