Yeast cell surface display technology has become an important tool in protein engineering research. By using this technology, protein interactions can be identified, protein affinity and specificity can be improved, protein stability and expression level can be increased, functional antigen maps can be drawn, and bioactive proteins and enzymes can be immobilized. The application of this technology represents the latest progress in protein engineering research.
Tumor is a common and frequently occurring disease. Currently, malignant tumors are the most serious threat to human health. For the treatment of malignant tumors, radiotherapy, chemotherapy, drug assisted therapy, and biological therapy are generally used. However, due to the proliferation, metastasis, and recurrence of malignant tumor cells, the treatment effect and prognosis are poor.
Yeast surface display is a powerful technique for separating and modifying proteins to enhance their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is influenced by secretion signals. In addition, the accessibility and activity of displayed proteins are influenced by the length of anchor proteins.
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