Yeast platform
The company focuses on yeast molecular biology technology as its research and development direction, and relies on independent innovative technology platforms such as "high-throughput yeast interaction technology", "yeast secretion expression", and "yeast display" to provide a full chain and scenario application solution for yeast
Explore SMART yeast library construction services at ProNet Biotech. Harness template-switching technology for efficient full-length mRNA cloning and low-abundance gene enrichment with high accuracy.
Yeast Two-Hybrid Screening Service (Membrane Protein)
Professional Membrane Yeast Two-Hybrid (MYTH) screening and validation service, designed for precise protein-protein interaction analysis. Benefit from cutting-edge split-ubiquitin technology, enhanced accuracy, and comprehensive insights through GO and KEGG analyses. Cost-effective, reliable, and tailored for researchers worldwide. Contact us today!
Yeast Two-Hybrid Screening Service (Nuclear Protein)
Professional Yeast Two-Hybrid (Y2H) screening and Validation service with nuclear protein cDNA libraries covering 100+ species free to use. Comprehensive protein-protein interaction analysis with NGS validation, GO/KEGG annotation, and expert support. Contact us to explore tailored solutions today!
Yeast One-Hybrid (Y1H) Screening Service
Explore ProNet Biotech's Yeast One-Hybrid (Y1H) Screening and Validation Services for precise DNA-protein interaction analysis. With fast turnaround, high-quality cDNA libraries, and comprehensive deliverables, our services empower your research into gene regulation and transcriptional networks. Contact us today!
Peptide Library Screening Service
Explore Pronetbio's high-throughput peptide library screening services using yeast two-hybrid (Y2H) technology to discover high-affinity peptide binders for protein interaction and therapeutic drug discovery.
Maize Transcription Factor Library
Explore the Maize Transcription Factor Library for efficient gene screening and protein interaction studies. This comprehensive collection features over 1,900 full-length cDNA clones from 81 transcription factor families in maize. Ideal for Yeast One-Hybrid (Y1H) and Yeast Two-Hybrid (Y2H) assays, gene function studies, and crop improvement research. Contact us for more information or to start your project.
CrY2H-seq Library Screening Service
Explore Pronetbio's advanced CrY2H-seq service for high-throughput protein-protein interaction screening using Cre-LoxP technology. Efficient, precise, and scalable, our service enables large-scale interaction discovery within yeast two-hybrid libraries. Contact ProNet Biotech for customized solutions and reliable results. Available worldwide.
TF-Centered Yeast One-Hybrid (Y1H-TF) Screening Service
Discover Pronetbio's TF-Centered Yeast One-Hybrid (Y1H-TF) Screening Service for efficient identification of transcription factor recognition elements. With a comprehensive yeast motif library, advanced bioinformatics integration, and rapid results around just one month, our service helps uncover core regulatory elements and supports gene regulatory network studies. Ideal for researchers seeking high-confidence motifs and cost-effective solutions. Contact us today to learn more.
Yeast Three-Hybrid (Y3H) Screening Service
Explore Yeast Three-Hybrid (Y3H) Screening for precise detection of protein-protein-small molecule interactions. Ideal for drug discovery, functional genomics, and signal pathway analysis. Accelerate your research with Service Code: SRY8011.
VHHs have several unique features including small size (15 kDa), high stability/solubility while retaining high binding affinity to their target antigens. VHHs are composed of 3 hypervariable regions, supporting antigen recognition against diverse targets and separated by 4 framework regions.
Our VHH scaffold is humanized for therapeutic applications reducing the level of immunogenicity. There is no animal use, thus antigens are not processed and VHHs can be developed against non-immunogenic or highly conserved proteins.
Our synthetic antibody library is a naive library with a complexity of 3.109 VHHs enabling to select highly functional conformational nanobodies and intrabodies.
Our Hybribody library possesses more diversity than in a herd of llamas! This is attributed to the rapid advances in synthetic biology and creative scientific efforts of our collaborators Drs. F. Perez and S. Moutel (Curie Institute, Paris) and Dr. A. Olichon (CRCT, Toulouse) (https://elifesciences.org/articles/16228).
Hybribody is a completely animal free antibody selection service. Discover here what our Lamas look like.
Intrabody Selection and Validation
Nanobodies for Cell Surface Antigens
Intrabody Selection and Validation
Optimization : Extracellular Affinity Maturation by Yeast Display
Optimization : Intracellular Affinity Maturation by Y2H
Minibody Cloning and Production
VHH for Immunofluorescence
VHH for Immunofluorescence
Our Ubiquitin Selectors with Nanotag®
Our optimized Phage Display selection technology delivers high quality and fully functional humanized recombinant nanobodies recognizing native antigens. Importantly, the structural integrity of the antigen is maintained throughout the entire VHH selection and validation process. Therefore, our nanobodies are more likely to recognize endogenous antigens for in vitro and in vivo applications, such as immunofluorescence, live cell imaging, protein degradation or blocking protein activity.
Key benefits
● Conformational antibodies
● Deliverables of the validated VHH binders include DNA sequences and plasmids
● Customized selection conditions (buffer, temperature, etc)
● Animal-free technology
Nanobodies are selected against membrane proteins or cell-surface antigens under native conditions.
The VHH candidates are directly selected on living cells by phage display. In this process, our proprietary library is incubated with the antigen expressed on cells. Subsequently, a washing step with antigen-free cells is performed and 3 to 4 rounds of selection follow.
Selected nanobodies are validated by immunofluorescence (IF) or phage-FACS assays. This selection strategy works very well for microbial antigens, such as expressed by parasites, bacteria or viruses.
Key benefits
● Variety of conformational nanobodies against a cell-surface target
Our intrabodies represent functional intracellular nanobodies expressed and properly folded in the cell cytoplasm where they recognize their endogenous target.
To enrich for intrabodies, we combine classical Phage Display Screening with our expertise in Yeast Two-Hybrid screening. These functional nanobodies are expressed inside the cell where they target endogenous antigen.
This combined strategy is useful for VHH selection against soluble antigens that are particularly difficult to express or purify in heterologous expression systems.
Key benefits
● Enrichment of intracellular targeting nanobodies
● No batch-to-batch variability
In vitro affinity maturation of nanobodies and scFv.
We use a combination of mutagenesis and yeast display to greatly improve the binding affinity of your nanobody or scFv. A mutant VHH or scFv library (2-5 x 106) derived from the parental sequence is generated by introducing mutations either across the entire length of the antibody sequence or restricted to the CDR regions and cloned into our proprietary vector for yeast surface expression.
Yeast expressing the mutant library is subjected to several rounds of cell sorting and the concentration of antigen is gradually decreased to select nanobodies with the highest binding affinities. Our yeast display platform controls for the antigen-antibody equilibrium, which is a major advantage compared to phage display.
Key benefits
● Cost-effective and quick optimization method
● No drift-effect like in phage display
● Apparent kD measurements
Maturation of VHH with our Yeast Two-Hybrid technology to obtain higher binding affinities for specifically intracellular applications (Intrabodization).
This service is divided into two phases: 1) creation of a mutant library generated by mutagenesis of the entire molecule or the CDR regions only 2) perform Y2H selection on the mutant library to identify intrabodies with increased binding affinities. The selection pressure is adjusted during the screening process to enrich for intrabodies with increased binding affinities.
Key benefits
● Cost-effective and rapid optimization process
● No-drift effect compared to phage display
Fuse your VHH with a Fc species of your choice to generate minibodies.
The selected VHH candidates are fused to Fc isotype-specific fragments from either human, mouse, sheep, rabbit, dog and rat to generate a “Minibody". This format is easily produced in CHO or HEK cells and can be used to stain antigens with standard antibody reagents.
Key benefits
● Versatile & flexible use for a vast array of applications (PLA, ELISA…)
● No batch-to-batch variability
● Possible fusion with Fc fragments from human, mouse, sheep, rabbit, dog and rat
● Enhanced avidity
To identify VHH candidates for immunofluorence (IF) applications, we express your antigen in cells as a fusion protein to a fluorescent tag which is targeted to a discrete subcellular localization. VHH-derived supernatants from E. coli are tested for co-localization by IF using standard secondary antibody reagents. This method enables the ranking of VHH candidates based on immunofluorescence intensity and co-localization analysis.
Key benefits
● ELISA & IF-validated VHH nanobody® in a single cDNA clone
● No variability from batch to batch
● Versatile tool thanks to the possible fusion with Fc fragments from human, mouse, sheep, rabbit, dog and rat
Ub-Selectors are the result from our Hybribody technology and are commercially available by Nanotag GmbH. These reagents are a perfect illustration of Hybribody nanobody solutions.
In general, ubiquitin is non-immunogenic and ubiquitin-chain specific nanobodies cannot be selected in animals. Therefore, our products represent a new entry point in the ubiquitin world and represent a new class of reagents that now can be developed.
FAQ
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Contact Us
Tel: +86 025-85205672
Email: info@pronetbio.com
Address: Building 3C, Nanjing Xianlin Zhigu,
Qixia District, Nanjing, Jiangsu, China, 210033
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